Purified anti-p53 Antibody
- 产品名称：
- Purified anti-p53 Antibody
- 产品类别：
- 抗体
- 产品编号：
- 645801
- 产品应用：
- 645801
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- p53 N-terminal linear epitope aa 20-25
- Formulation
- This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- This antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
ICC, ChIP - Verified
ELISA - Reported in the literature, not verified in house
- Recommended Usage
Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 0.5 - 1.0 ?g per mL?antibody for each mini-gel.?For immunocytochemistry, a concentration range of 2.0 - 5.0 μg/mL?(Dilution 1:100 - 1:250) is recommended.?The recommended starting antibody to chromatin ratio is 1 ?g of antibody and 5 ?g of chromatin per IP. Optimal antibody to chromatin ratio should be determined by the end user. It is recommended that the reagent be titrated for optimal performance for each application.
- Application References
(PubMed link indicates BioLegend citation) -
- Vojtesek B, et al. 1992. J. Immunol. Methods 151:237.
- Stephen CW, et al. 1995. J. Mol. Biol. 248:58.
- Product Citations
-
- Senosain MF, et al. 2021. Sci Rep. 11:14424. PubMed
- RRID
- AB_2206461 (BioLegend Cat. No. 645801) AB_2206460 (BioLegend Cat. No. 645802)
- Structure
- Tetramer; 53 kD
- Distribution
-
Nuclear when activated
- Function
- Tumor suppressor, transcription factor regulates cell cycle checkpoint, apoptosis, DNA integrity
- Interaction
- mdm2, mdmx, TopoI, PML3, Bcl-XL, Bcl-2, Chk1, JNK, p38, HIPK2, CK2, DNA-PK, p300/CBP, PCAF, PARP1, HDAC1-3; mutant p53 associates with p63, p73
- Cell Type
- Mesenchymal Stem Cells
- Biology Area
- Cell Biology, DNA Repair/Replication, Stem Cells
- Gene ID
- 7157 View all products for this Gene ID
- UniProt
- View information about p53 on UniProt.org
