Purified anti-p53 Antibody -上海雅吉生物科技有限公司

规格	价格	库存
25µg	￥ 1484	1

Product Details

Verified Reactivity

Human

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

P53 N-terminal linear epitope aa 20-25

Formulation

This antibody is provided in phosphate-buffered solution containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography.

Concentration

0.5 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C.

Application

WB - Quality tested
ICC, ChIP - Verified
IP - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 0.5 - 1 ?g per ml antibody for each mini-gel.?For immunocytochemistry, a concentration range of 2.0 - 5.0 μg/ml (Dilution 1:100 - 1:250) is recommended. For ChIP applications, the recommended starting antibody to chromatin ratio is 1 ?g of antibody and 5 ?g of chromatin per IP. Optimal antibody to chromatin ratio should be determined by the end user. It is recommended that the reagent be titrated for optimal performance for each application.

Application References (PubMed link indicates BioLegend citation)

Vojtesek B, et al. 1992. J Immuno Methods 151:237
Stephen CW, et al. 1995. J Mol Biol 248:58

Product Citations

Geisinger JM, et al. 2020. Nucleic Acids Res. 48:9067. PubMed
Linetsky M, et al. 2020. Free Radic Biol Med. 152:280. PubMed
Lim J, et al. 2021. Curr Protoc. 1:e29. PubMed

RRID

AB_1595606 (BioLegend Cat. No. 645701) AB_2206462 (BioLegend Cat. No. 645702)

Antigen Details

Structure: Tetramer; 53 kD
Distribution: Nuclear when activated
Function: Tumor suppressor, transcription factor regulates cell cycle checkpoint, apoptosis, DNA integrity
Interaction: mdm2, mdmx, TopoI, PML3, Bcl-X_L, Bcl-2, Chk1, JNK, p38, HIPK2, CK2, DNA-PK, p300/CBP, PCAF, PARP1, HDAC1-3; mutant p53 associates with p63, p73
Cell Type: Mesenchymal Stem Cells
Biology Area: Cell Biology, DNA Repair/Replication, Stem Cells, Transcription Factors
Antigen References: 1. Vogelstein B, et al. 1992. Cell. 70:523.
2. Shieh S, et al. 1997. Cell. 91:325.
3. Mihara M, et al. 2003. Mol. Cell 11:577.
4. Saito S, et al. 2003. J. Biol. Chem. 278:37536.
Gene ID: 7157 View all products for this Gene ID
UniProt: View information about p53 on UniProt.org

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