Purified anti-human CD138 (Syndecan-1) Antibody
- 产品名称:
- Purified anti-human CD138 (Syndecan-1) Antibody
- 产品类别:
- 抗体
- 产品编号:
- 352301
- 产品应用:
- 352301
- Verified Reactivity
- Human
- Reported Reactivity
- African Green, Baboon, Cynomolgus
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Human SDC1
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
FC -?Quality tested
IHC-P -?Verified
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 ?g per million cells in 100 ?l volume. For immunohistochemical staining on formalin-fixed paraffin-embedded tissue sections, the suggested use of this reagent is 5 - 10 ?g per ml.?It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
The epitope recognized by MI15 is found within the ectodomain of the CD138 core protein. It has been reported that MI15 blocks the binding of clone B-B4 but not clone DL-101 by flow cytometric analysis. Clones DL-101 and MI15 exhibit differential staining patterns on in vitro generated plasma cells and some CD138+ cell lines.2
Additional reported applications (for the relevant formats of this clone) include: immunohistochemical staining in paraffin blocks of tissue sections1.
- Application References
(PubMed link indicates BioLegend citation) -
- Osama MA. 2010. Int. J. Clin. Exp. Pathol. 3:280. (IHC)
- Itoua MR, et al. 2014. Biomed. Res. Int. 2014:536482. PubMed
- Product Citations
-
- Kaufmann M, et al. 2021. Med. 2(3):296-312.e8. PubMed
- Yang Z, et al. 2021. Sci Adv. 7:. PubMed
- RRID
- AB_10915557 (BioLegend Cat. No. 352301) AB_10915555 (BioLegend Cat. No. 352302)
- Structure
- 100-200 kD type I integral transmembrane glycoprotein
- Distribution
-
Plasma cells, pre-B cells, epithelial cells, endothelial cells
- Function
- Adhesion, control cell morphology, regulate cell growth
- Ligand/Receptor
- FGFb, collagen, fibronectin
- Cell Type
- B cells, Endothelial cells, Epithelial cells, Plasma cells
- Biology Area
- Cell Adhesion, Cell Biology, Immunology
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
-
1. Sanderson RD, et al. 1992. Cell. Regul. 1:27.
2. Zola H, et al. 2007. Leukocyte and Stromal Cell Molecules: The CD Markers Wiley-Liss A John Wiley & Sons Inc, Publication.
- Gene ID
- 6382 View all products for this Gene ID
- UniProt
- View information about CD138 on UniProt.org