Purified anti-human CD262 (DR5, TRAIL-R2) Antibody
- 产品名称:
- Purified anti-human CD262 (DR5, TRAIL-R2) Antibody
- 产品类别:
- 抗体
- 产品编号:
- 307302
- 产品应用:
- 307302
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Extracellular domain of DR5-human IgG1 Fc fusion protein
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 2.0 ?g per million cells in 100 ?l volume or 100 ?l of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
Additional reported applications (for the relevant formats) include: The DJR2-2 antibody is useful for immunofluorescent staining and flow cytometric analysis of DR5/TRAIL-R2 receptor expression. For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated anti-mouse IgG second step (Cat. No. 405303), followed by SAv-PE (Cat. No. 405204)).
- Application References
(PubMed link indicates BioLegend citation) -
- Uno K, et al. 2003. Blood 101:3658.
- Sato K, et al. 2005. J. Immunol. 174:4025.
- Shi J, et al. 2008. Blood 111:1309.PubMed
- Sonnemann J, et al. 2012. Cancer Biol Ther. 13:417. PubMed
- Product Citations
-
- Vunnam N, et al. 2020. Biochemistry. 59:3856. PubMed
- Winterberg D, et al. 2021. J Clin Med. 10:. PubMed
- Sonnemann J, et al. 2012. Cancer Biol Ther. 13:417. PubMed
- Mitchell M, et al. 2017. Nat Commun. 10.1038/ncomms14179. PubMed
- Shi J, et al. 2008. Blood. 111:1309. PubMed
- RRID
- AB_314672 (BioLegend Cat. No. 307302)
- Structure
- TNFR superfamily, 55 kD
- Distribution
- Broadly expressed on normal and tumor cells
- Function
- Induces apoptosis
- Ligand/Receptor
- TRAIL
- Biology Area
- Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology
- Molecular Family
- CD Molecules, Cytokine/Chemokine Receptors
- Antigen References
-
1. MacFarlane M, et al. 1997. J. Biol. Chem. 272:25417.
2. Walczak H, et al. 1997. EMBO J. 16:5386.
3. Shigeno M, et al. 2003. Oncogene 22:1653.
4. LaVallee T, et al. 2003. Cancer Res. 63:468.
5. Nimmanapalli R, et al. 2001. Cancer Res. 61:759
- Gene ID
- 8795 View all products for this Gene ID
- UniProt
- View information about CD262 on UniProt.org