Ultra-LEAF™ Purified anti-mouse IL-33R&alpha
- 产品名称：
- Ultra-LEAF™ Purified anti-mouse IL-33R&alpha
- 产品类别：
- 抗体
- 产品编号：
- 146604
- 产品应用：
- 146604
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- IL-33Rα-hFcγ 1 fusion protein
- Formulation
- 0.2 ?m filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is <0.01 EU/?g of the protein (<0.001 ng/?g of the protein) as determined by the LAL test.
- Preparation
- The Ultra-LEAF? (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
- Concentration
- The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF? solution contains no preservative; handle under aseptic conditions.
- Application
-
FC - Quality tested
Block - Verified
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 ?g per million cells in 100 ?l volume or 100 ?l of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
- Application References
(PubMed link indicates BioLegend citation) -
- Hashiguchi M, et al. 2014. Eur. J. Immunology. (FC, Block) PubMed
- Product Citations
-
- Hoppe A, et al. 2020. J Allergy Clin Immunol. 1476:145. PubMed
- Suzuki Y, et al. 2021. FEBS Open Bio. 11:2619. PubMed
- RRID
- AB_2562656 (BioLegend Cat. No. 146604)
- Structure
- Approximately 63kD, IL-1 receptor family member, single pass type I membrane protein
- Distribution
-
Mast cells, Th2 cells and fibroblasts
- Function
- Interacts with IL-33 and recruits MyD88, Irak1, Irak4 and Traf6 followed by phosphorylation of the MAP/ERK pathway
- Interaction
- Interacts with MyD88, Irak1, Irak4 and Traf6
- Ligand/Receptor
- IL-33
- Cell Type
- Mast cells, Th2, Fibroblasts
- Biology Area
- Immunology, Innate Immunity
- Molecular Family
- Cytokine/Chemokine Receptors
- Antigen References
-
- Yanagisawa K, et al. 1993. FEBS Lett. 318:83.
- Schmitt E,?et al. 1990. Cytokine. 6:407.
- Yanagisawa K, et al. 1992. FEBS Lett. 302:51.
- Takagi T,?et al. 1993. Biochim. Biophys. Acta. 1178:194.
- Gene ID
- 17082 View all products for this Gene ID
- UniProt
- View information about IL-33Ralpha on UniProt.org
