Biotin anti-human Granulysin Antibody -上海雅吉生物科技有限公司

规格	价格	库存
50µg	￥ 2464	1

Product Details

Verified Reactivity

Human

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

Recombinant 9 kD granulysin

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography and conjugated with biotin under optimal conditions.

Concentration

0.5 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.

Application

ELISA Detection - Quality tested
Neut, IP - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by ELISA assay. A concentration of 1 ?g/?l of the detection antibody was utilized to gernerate the example standard curve. It is recommended that the end-user titrate each lot of reagents for optimal performance.

Application Notes

ELISA Detection: To measure human granulysin, this antibody can be used as a detection antibody in sandwich ELISA format and paired with the purified DH2 antibody (Cat. No. 348007) as the capture antibody.
Additional reported applications (for the relevant formats) include: neutralization¹ and immunoprecipitation¹.
Note: For testing human granulysin in serum, plasma or cell culture supernatant, LEGEND MAX? Human Granulysin ELISA Kit with Pre-coated Plates (Cat. No. 438007 & 438008) are specially developed and recommended.

Application References (PubMed link indicates BioLegend citation)

Tewari P, et al. 2010. Blood. 116:3465. (Neut, IP)

RRID

AB_2563603 (BioLegend Cat. No. 526103) AB_2563604 (BioLegend Cat. No. 526104)

Antigen Details

Structure: Saposin-like protein family of lipid binding protein, 9 kD and 15 kD
Distribution: NK cells, cytolytic T cells
Function: Cytolytic against bacteria, fungi, parasite and tumor cells; chemoattract inflammatory cells
Cell Type: Neutrophils, NK cells, T cells
Biology Area: Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology
Molecular Family: Cytokines/Chemokines
Antigen References: 1. Hanson DA, et al. 1999. Mol. Immunol. 36:413.
2. Spada FM, et al. 2000. J. Exp. Med. 191:937.
3. Kalb ML, et al. 2012. J. Immunol. 188:1583.
Gene ID: 10578 View all products for this Gene ID
UniProt: View information about Granulysin on UniProt.org

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