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Purified anti-human Perforin Antibody
产品名称:
Purified anti-human Perforin Antibody
产品类别:
抗体
产品编号:
308102
产品应用:
308102
[价格]
规格 价格 库存
100µg ¥ 2884 1

产品详情

Product Details

Verified Reactivity
Human
Reported Reactivity
Cow
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Purified granules from the human lymphoma cell line
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

ICFC - Quality tested
IP, ICC, IHC - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.06 ?g per million cells in 100 ?L?volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Clone dG9 primarily recognizes perforin associated with cytotoxic granules9. Additional reported applications (for the relevant formats) include: immunoprecipitation, intracellular flow cytometric analysis and immunofluorescence microscopy5,7, and immunohistochemical staining of acetone-fixed frozen tissue sections and formalin-fixed paraffin-embedded tissue sections1,4.


Does not cross-react with mouse1.

Application References

(PubMed link indicates BioLegend citation)
  1. Hameed A, et al. 1992. Am. J. Pathol. 140:1025. (IHC)
  2. Schaerli P, et al. 2004. J. Exp. Med. 199:1265.
  3. Watanabe N, et al. 1997. Blood 90:3662.
  4. Mauad T, et al. 2004. Pediatr. Pulmonol. 38:233. (IHC)
  5. Barrat FJ, et al. 1999. P. Natl. Acad. Sci. USA 96:8645. (IF)
  6. Chen H, et al. 2005. J. Immunol. 175:591.
  7. Bryceson YT, et al. 2007. Blood doi:10.1182/blood-2007-02-074468. (IF)
  8. Wood SM, et al. 2009. Blood 114:4117. PubMed
  9. Makedonas G, et al. 2010. PLoS Pathog. 6:e1000798.
Product Citations
  1. Trinks N, et al. 2021. Commun Biol. 4:1151. PubMed
  2. Crespo ?C, et al. 2020. Cell. 182(5):1125-1139. PubMed
  3. Dean JW, et al. 2020. J Autoimmun. 108:102417. PubMed
  4. Mann ER, et al. 2020. Sci Immunol. :5. PubMed
  5. Del Alcazar D, et al. 2019. Cell Rep. 28:3047. PubMed
  6. Mace E, Orange J 2014. J Vis Exp. 85: 51100. PubMed
  7. Wood S, et al. 2009. Blood. 114:4117. PubMed
RRID
AB_314700 (BioLegend Cat. No. 308102)

Antigen Details

Structure
70 kD
Distribution

CTL, NK (cytoplasmic granules)

Function
Mediates targeted cell lysis
Cell Type
NK cells, T cells
Biology Area
Cell Biology, Immunology, Innate Immunity, Neuroscience
Molecular Family
Cytokines/Chemokines
Antigen References

1. Lieberman J. 2003. Nat. Rev. Immunol. 3:361.
2. Trapani J, et al. 2002. Nat. Rev. Immunol. 2:735.

Gene ID
5551 View all products for this Gene ID
UniProt
View information about Perforin on UniProt.org

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