Pacific Blue™ anti-human Perforin Antibody -上海雅吉生物科技有限公司

规格	价格	库存
25tests	￥ 2058	1

Product Details

Verified Reactivity

Human

Reported Reactivity

Cow

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

Purified granules from the human lymphoma cell line

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)

Preparation

The antibody was purified by affinity chromatography, and conjugated with Pacific Blue? under optimal conditions.

Concentration

Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

ICFC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood.

* Pacific Blue? has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue? conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.

Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.

View full statement regarding label licenses

Excitation Laser

Violet Laser (405 nm)

Application Notes

Clone dG9 primarily recognizes perforin associated with cytotoxic granules⁹. Additional reported applications (for the relevant formats) include: immunoprecipitation, intracellular flow cytometric analysis and immunofluorescence microscopy^5,7, and immunohistochemical staining of acetone-fixed frozen tissue sections and formalin-fixed paraffin-embedded tissue sections^1,4.

Does not cross-react with mouse¹.

Application References (PubMed link indicates BioLegend citation)

Hameed A, et al. 1992. Am. J. Pathol. 140:1025. (IHC)
Schaerli P, et al. 2004. J. Exp. Med. 199:1265.
Watanabe N, et al. 1997. Blood 90:3662.
Mauad T, et al. 2004. Pediatr. Pulmonol. 38:233. (IHC)
Barrat FJ, et al. 1999. P. Natl. Acad. Sci. USA 96:8645. (IF)
Chen H, et al. 2005. J. Immunol. 175:591.
Bryceson YT, et al. 2007. Blood doi:10.1182/blood-2007-02-074468. (IF)
Wood SM, et al. 2009. Blood 114:4117. PubMed
Makedonas G, et al. 2010. PLoS Pathog. 6:e1000798.

Product Citations

Schmitz T, et al. 2021. J Reprod Immunol. 148:103424. PubMed
Crespo ?C, et al. 2020. Cell. 182(5):1125-1139. PubMed
Spandan V Shah et al. 2018. Cell reports. 25(10):2766-2774 . PubMed
Manickam C, et al. 2016. J Virol. 90: 9153 - 9162. PubMed
Bhattacharya P, et al. 2020. Elife. 9:00. PubMed

RRID

AB_10895768 (BioLegend Cat. No. 308117) AB_10899565 (BioLegend Cat. No. 308118)

Antigen Details

Structure: 70 kD
Distribution: CTL, NK (cytoplasmic granules)
Function: Mediates targeted cell lysis
Cell Type: NK cells, T cells
Biology Area: Cell Biology, Immunology, Innate Immunity, Neuroscience
Molecular Family: Cytokines/Chemokines
Antigen References: 1. Lieberman J. 2003. Nat. Rev. Immunol. 3:361.
2. Trapani J, et al. 2002. Nat. Rev. Immunol. 2:735.
Gene ID: 5551 View all products for this Gene ID
UniProt: View information about Perforin on UniProt.org

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