Brilliant Violet 421™ anti-human Perforin An-上海雅吉生物科技有限公司

规格	价格	库存
25tests	￥ 2548	1

Product Details

Verified Reactivity

Human

Reported Reactivity

Cow

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

Purified granules from the human lymphoma cell line

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).

Preparation

The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421? under optimal conditions.

Concentration

Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

ICFC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood.

Brilliant Violet 421? excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421? is a trademark of Sirigen Group Ltd.

Learn more about Brilliant Violet?.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.

Excitation Laser

Violet Laser (405 nm)

Application Notes

Clone dG9 primarily recognizes perforin associated with cytotoxic granules⁹. Additional reported applications (for the relevant formats) include: immunoprecipitation, intracellular flow cytometric analysis and immunofluorescence microscopy^5,7, and immunohistochemical staining of acetone-fixed frozen tissue sections and formalin-fixed paraffin-embedded tissue sections^1,4.

Does not cross-react with mouse¹.

Application References (PubMed link indicates BioLegend citation)

Hameed A, et al. 1992. Am. J. Pathol. 140:1025. (IHC)
Schaerli P, et al. 2004. J. Exp. Med. 199:1265.
Watanabe N, et al. 1997. Blood 90:3662.
Mauad T, et al. 2004. Pediatr. Pulmonol. 38:233. (IHC)
Barrat FJ, et al. 1999. P. Natl. Acad. Sci. USA 96:8645. (IF)
Chen H, et al. 2005. J. Immunol. 175:591.
Bryceson YT, et al. 2007. Blood doi:10.1182/blood-2007-02-074468. (IF)
Wood SM, et al. 2009. Blood 114:4117. PubMed
Makedonas G, et al. 2010. PLoS Pathog. 6:e1000798.

Product Citations

Park SY, et al. 2022. NPJ Vaccines. 7:123. PubMed
Caduff N, et al. 2021. Cell Reports. 35(5):109056. PubMed
Hubrack S, et al. 2021. Sci Rep. 11:14090. PubMed
Singh KS, et al. 2021. Nature. 589:597. PubMed
Seo IH, et al. 2021. Cell Reports. 36(4):109438. PubMed
Chen YP, et al. 2020. Cell Res. 30:1024. PubMed

RRID

AB_2566203 (BioLegend Cat. No. 308121) AB_2566204 (BioLegend Cat. No. 308122)

Antigen Details

Structure: 70 kD
Distribution: CTL, NK (cytoplasmic granules)
Function: Mediates targeted cell lysis
Cell Type: NK cells, T cells
Biology Area: Cell Biology, Immunology, Innate Immunity, Neuroscience
Molecular Family: Cytokines/Chemokines
Antigen References: 1. Lieberman J. 2003. Nat. Rev. Immunol. 3:361.
2. Trapani J, et al. 2002. Nat. Rev. Immunol. 2:735.
Gene ID: 5551 View all products for this Gene ID
UniProt: View information about Perforin on UniProt.org

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