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首页 > 抗体蛋白 > 抗体
Alexa Fluor® 647 anti-human Perforin Antibody
产品名称：
Alexa Fluor® 647 anti-human Perforin Antibody
产品类别：
抗体
产品编号：
308109
产品应用：
308109
[价格]
规格 价格 库存
25tests ￥ 1736 1

产品详情

Product Details

Verified Reactivity
Human
Reported Reactivity
Cow
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Purified granules from the human lymphoma cell line
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 647 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?L?per million cells in 100 ?L?staining volume or 5 ?L?per 100 ?L?of whole blood.

* Alexa Fluor? 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm.


Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.

View full statement regarding label licenses
Excitation Laser
Red Laser (633 nm)
Application Notes

Clone dG9 primarily recognizes perforin associated with cytotoxic granules9. Additional reported applications (for the relevant formats) include: immunoprecipitation, intracellular flow cytometric analysis and immunofluorescence microscopy5,7, and immunohistochemical staining of acetone-fixed frozen tissue sections and formalin-fixed paraffin-embedded tissue sections1,4.


Does not cross-react with mouse1.

Application References

(PubMed link indicates BioLegend citation)
  1. Hameed A, et al. 1992. Am. J. Pathol. 140:1025. (IHC)
  2. Schaerli P, et al. 2004. J. Exp. Med. 199:1265.
  3. Watanabe N, et al. 1997. Blood 90:3662.
  4. Mauad T, et al. 2004. Pediatr. Pulmonol. 38:233. (IHC)
  5. Barrat FJ, et al. 1999. P. Natl. Acad. Sci. USA 96:8645. (IF)
  6. Chen H, et al. 2005. J. Immunol. 175:591.
  7. Bryceson YT, et al. 2007. Blood doi:10.1182/blood-2007-02-074468. (IF)
  8. Wood SM, et al. 2009. Blood 114:4117. PubMed
  9. Makedonas G, et al. 2010. PLoS Pathog. 6:e1000798.
Product Citations
  1. Kim N, et al. 2022. Front Immunol. 12:792334. PubMed
  2. Bezverbnaya K, et al. 2021. Cytotherapy. 23:820. PubMed
  3. Sevilya Z, et al. 2018. Front Immunol. 9:2068. PubMed
  4. Pérez-Quintero L, et al. 2014. J Exp Med. 211:727. PubMed
  5. Bryceson Y, et al. 2007. Blood. 110:1906. PubMed
  6. Kim N, et al. 2017. Int J Mol Sci. 10.3390/ijms18061262. PubMed
  7. Orange E 2014. Proc Natl Acad Sci U S A. 111:6708. PubMed
  8. Blandine Monel et al. 2019. Cell reports. 27(1):142-153 . PubMed
  9. Peruzzi G, et al. 2013. J Immunol. 191:1883. PubMed
  10. Krzewski K, et al. 2008. Proc Natl Acad Sci U S A. 105:2568. PubMed
  11. Rak G, et al. 2011. PLoS One. 6:e1001151. PubMed
  12. Qu B, et al. 2011. J Immunol. 186:6894. PubMed
  13. Li Y, et al. 2022. J Immunol. 208:347. PubMed
  14. German Y, et al. 2021. Cell Reports. 36(1):109318. PubMed
  15. Chitirala P, et al. 2020. eLife. 9:00. PubMed
  16. Sanchez-Ruiz Y, et al. 2011. PLoS One. 6:e27057. PubMed
  17. Xie CB, et al. 2020. J Clin Invest. 130:3437. PubMed
  18. Salzer E, et al. 2016. Nat Immunol. 17:1352-1360. PubMed
  19. Dutertre C, et al. 2008. J Leukoc Biol. 84:1511. PubMed
  20. Khazen R, et al. 2016. Nat Commun. 7:10823. PubMed
RRID
AB_493255 (BioLegend Cat. No. 308109) AB_493254 (BioLegend Cat. No. 308110)

Antigen Details

Structure
70 kD
Distribution

CTL, NK (cytoplasmic granules)

Function
Mediates targeted cell lysis
Cell Type
NK cells, T cells
Biology Area
Cell Biology, Immunology, Innate Immunity, Neuroscience
Molecular Family
Cytokines/Chemokines
Antigen References

1. Lieberman J. 2003. Nat. Rev. Immunol. 3:361.
2. Trapani J, et al. 2002. Nat. Rev. Immunol. 2:735.

Gene ID
5551 View all products for this Gene ID
UniProt
View information about Perforin on UniProt.org

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