Brilliant Violet 421™ anti-mouse/human CD324-上海雅吉生物科技有限公司

规格	价格	库存
50µg	￥ 3542	1

Product Details

Verified Reactivity

Mouse, Human

Reported Reactivity

Cynomolgus, Dog, Pig

Antibody Type

Monoclonal

Host Species

Rat

Immunogen

E-Cadherin extracellular domain

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).

Preparation

The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421? under optimal conditions.

Concentration

0.2 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC -?Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 421? excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421? is a trademark of Sirigen Group Ltd.

Learn more about Brilliant Violet?.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.

Excitation Laser

Violet Laser (405 nm)

Application Notes

Additional reported applications (for relevant formats) include: immunoprecipitation¹, Western Blotting¹, immunomicroscopy³, biological function^1,2, and spatial biology (IBEX)^4,5.

Application References (PubMed link indicates BioLegend citation)

Vestweber D, et al. 1985. EMBO. 4:3393. (IP, WB, FA)
Nakagawa M, et al. 2001. J. Cell Sci. 114:1829. (FA in canine cells)
Mohamet L, et al. 2010. PLoS ONE. 5:e12921. (IF)
Radtke?AJ,?et al.?2020.?Proc Natl Acad Sci U S A.?117:33455-65. (SB)?PubMed
Radtke?AJ,?et al.?2022.?Nat Protoc.?17:378-401. (SB)?PubMed

Product Citations

Khodeer S, et al. 2022. J Cell Sci. 135:. PubMed
Bernardini C, et al. 2021. Animals (Basel). 11:. PubMed
Norgard RJ, et al. 2021. EMBO Rep. 22:e51872. PubMed

RRID

AB_2750483 (BioLegend Cat. No. 147319)

Antigen Details

Structure: Member of the cadherin superfamily. Calcium-dependent, transmembrane cell-cell adhesion glycoprotein composed of four extracellular cadherin repeats and a highly conserved cytoplasmic tail region.
Distribution: Widely expressed in epithelial cells in the colon, uterus, liver, keratinocytes, brain, heart, muscle, kidney, and pancreas as well as erythroid cells.
Function: Cell adhesion molecule involved in development, bacterial pathogenesis, and tumor invasion. The ectodomain of CD324 mediates bacterial adhesion to mammalian cells, while the cytoplasmic domain is required for internalization.
Interaction: Interacts with a variety of proteins including erbin, ezrin, caspase-3, caspase-8, EGF receptor, β-catenin, presenilin 1, casein kinase II, and others.
Ligand/Receptor: αEβ7 integrin.
Cell Type: Embryonic Stem Cells
Biology Area: Cell Adhesion, Cell Biology, Immunology, Innate Immunity, Neuroscience, Stem Cells, Synaptic Biology
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Overduin M, et al. 1995. Science 267:386.
2. Boggon TJ, et al. 2002. Science 296:1308.
3. Berx G, et al. 1995. EMBO J. 14:6107.
4. Perl AK, et al. 1998. Nature 392:190.
Gene ID: 999 View all products for this Gene ID 12550 View all products for this Gene ID
UniProt: View information about CD324 on UniProt.org

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