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Alexa Fluor® 647 anti-mouse/human CD324 (E-Cad
产品名称：
Alexa Fluor® 647 anti-mouse/human CD324 (E-Cad
产品类别：
抗体
产品编号：
147307
产品应用：
147307
[价格]
规格 价格 库存
25µg ￥ 1736 1

产品详情

Product Details

Verified Reactivity
Mouse, Human
Reported Reactivity
Cynomolgus, Dog, Pig
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
E-Cadherin extracellular domain
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 647 under optimal conditions.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
ICC, IHC-F, 3D IHC?- Verified

SB - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 ?g per million cells in 100 ?L?volume. For immunocytochemistry, a concentration range of 2.0 - 10 μg/mL is recommended.?For immunohistochemical staining on frozen tissue sections, a concentration range of 5.0 - 10 ?g/mL?is suggested. For 3D immunohistochemistry on formalin-fixed tissues, a concentration of 5.0 ?g/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor? 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.


Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.

View full statement regarding label licenses
Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for relevant formats) include: immunoprecipitation1, Western Blotting1, immunomicroscopy3, biological function1,2, and spatial biology (IBEX)4,5.

Additional Product Notes

Iterative Bleaching Extended multi-pleXity (IBEX) is a fluorescent imaging technique capable of highly-multiplexed spatial analysis. The method relies on cyclical bleaching of panels of fluorescent antibodies in order to image and analyze many markers over multiple cycles of staining, imaging, and, bleaching. It is a community-developed open-access method developed by the Center for Advanced Tissue Imaging (CAT-I) in the National Institute of Allergy and Infectious Diseases (NIAID, NIH).

Application References

(PubMed link indicates BioLegend citation)
  1. Vestweber D, et al. 1985. EMBO. 4:3393. (IP, WB, FA)
  2. Nakagawa M, et al. 2001. J. Cell Sci. 114:1829. (FA in canine cells)
  3. Mohamet L, et al. 2010. PLoS ONE. 5:e12921. (IF)
  4. Radtke?AJ,?et al.?2020.?Proc Natl Acad Sci U S A.?117:33455-65. (SB)?PubMed
  5. Radtke?AJ,?et al.?2022.?Nat Protoc.?17:378-401. (SB)?PubMed
Product Citations
  1. Khodeer S, et al. 2022. J Cell Sci. 135:. PubMed
  2. Law NC, et al. 2019. Nat Commun. 10:2787. PubMed
  3. Rodríguez E, et al. 2022. Commun Biol. 5:41. PubMed
  4. Pitarresi JR, et al. 2021. Cancer Discov. 11:1774. PubMed
  5. Cohen SB et al. 2018. Cell host & microbe. 24(3):439-446 . PubMed
  6. Stefkovich ML, et al. 2021. Curr Protoc. 1:e139. PubMed
  7. Gentek R, et al. 2018. Immunity. 48:1160. PubMed
  8. Han S, et al. 2019. Cell Stem Cell. 25:342. PubMed
  9. Rosàs-Canyelles E, et al. 2020. Sci Adv. 6:eaay1751. PubMed
  10. Emily A Thompson et al. 2019. Cell reports. 26(11):2859-2867 . PubMed
  11. Steele NG, et al. 2021. Clin Cancer Res. 27:2023. PubMed
  12. Norgard RJ, et al. 2021. EMBO Rep. 22:e51872. PubMed
  13. Gawronska-Kozak B, et al. 2016. PLoS One. 11: 0150635. PubMed
  14. Malenica I, et al. 2021. Nat Commun. 12:5209. PubMed
  15. Radke J, et al. 2022. Nat Commun. 13:7304. PubMed
RRID
AB_2563954 (BioLegend Cat. No. 147307) AB_2563955 (BioLegend Cat. No. 147308)

Antigen Details

Structure
Member of the cadherin superfamily. Calcium-dependent, transmembrane cell-cell adhesion glycoprotein composed of four extracellular cadherin repeats and a highly conserved cytoplasmic tail region.
Distribution

Widely expressed in epithelial cells in the colon, uterus, liver, keratinocytes, brain, heart, muscle, kidney, and pancreas as well as erythroid cells.

Function
Cell adhesion molecule involved in development, bacterial pathogenesis, and tumor invasion. The ectodomain of CD324 mediates bacterial adhesion to mammalian cells, while the cytoplasmic domain is required for internalization.
Interaction
Interacts with a variety of proteins including erbin, ezrin, caspase-3, caspase-8, EGF receptor, β-catenin, presenilin 1, casein kinase II, and others.
Ligand/Receptor
αEβ7 integrin.
Cell Type
Embryonic Stem Cells
Biology Area
Cell Adhesion, Cell Biology, Immunology, Innate Immunity, Neuroscience, Stem Cells, Synaptic Biology
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Overduin M, et al. 1995. Science 267:386.
2. Boggon TJ, et al. 2002. Science 296:1308.
3. Berx G, et al. 1995. EMBO J. 14:6107.
4. Perl AK, et al. 1998. Nature 392:190.

Gene ID
999 View all products for this Gene ID 12550 View all products for this Gene ID
UniProt
View information about CD324 on UniProt.org

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