Alexa Fluor® 594 anti-mouse/human CD324 (E-Cad-上海雅吉生物科技有限公司

规格	价格	库存
100µg	￥ 2884	1

Product Details

Verified Reactivity

Mouse, Human

Reported Reactivity

Cynomolgus, Dog, Pig

Antibody Type

Monoclonal

Host Species

Rat

Immunogen

E-Cadherin extracellular domain

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 594 under optimal conditions.

Concentration

0.5 mg/mL

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

ICC - Quality tested
IHC-P, IHC-F, 3D IHC - Verified

Recommended Usage

Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a concentration range of 5.0 - 20 μg/mL?is recommended.?For immunohistochemical staining of formalin-fixed paraffin-embedded tissue sections, a concentration range of 5.0 - 10 μg/mL?is suggested. For immunohistochemical staining of frozen tissue sections, a concentration range of 2.0 - 5.0 μg/mL?is suggested. For 3D immunohistochemistry on formalin-fixed tissues, a concentration of 5.0 ?g/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor? 594 has an excitation maximum of 590 nm, and a maximum emission of 617 nm.

Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.

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Application Notes

Additional reported applications (for relevant formats) include: immunoprecipitation¹, Western Blotting¹, immunomicroscopy³, biological function^1,2, and spatial biology (IBEX)^4,5.

Application References (PubMed link indicates BioLegend citation)

Vestweber D, et al. 1985. EMBO. 4:3393. (IP, WB, FA)
Nakagawa M, et al. 2001. J. Cell Sci. 114:1829. (FA in canine cells)
Mohamet L, et al. 2010. PLoS ONE. 5:e12921. (IF)
Radtke?AJ,?et al.?2020.?Proc Natl Acad Sci U S A.?117:33455-65. (SB)?PubMed
Radtke?AJ,?et al.?2022.?Nat Protoc.?17:378-401. (SB)?PubMed

Product Citations

Zhang X, et al. 2019. iScience. 19:607. PubMed
Moon H, et al. 2019. Nat Commun. 10:2225. PubMed

RRID

AB_2563230 (BioLegend Cat. No. 147306)

Antigen Details

Structure: Member of the cadherin superfamily. Calcium-dependent, transmembrane cell-cell adhesion glycoprotein composed of four extracellular cadherin repeats and a highly conserved cytoplasmic tail region.
Distribution: Widely expressed in epithelial cells in the colon, uterus, liver, keratinocytes, brain, heart, muscle, kidney, and pancreas as well as erythroid cells.
Function: Cell adhesion molecule involved in development, bacterial pathogenesis, and tumor invasion. The ectodomain of CD324 mediates bacterial adhesion to mammalian cells, while the cytoplasmic domain is required for internalization.
Interaction: Interacts with a variety of proteins including erbin, ezrin, caspase-3, caspase-8, EGF receptor, β-catenin, presenilin 1, casein kinase II, and others.
Ligand/Receptor: αEβ7 integrin.
Cell Type: Embryonic Stem Cells
Biology Area: Cell Adhesion, Cell Biology, Immunology, Innate Immunity, Neuroscience, Stem Cells, Synaptic Biology
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Overduin M, et al. 1995. Science 267:386.
2. Boggon TJ, et al. 2002. Science 296:1308.
3. Berx G, et al. 1995. EMBO J. 14:6107.
4. Perl AK, et al. 1998. Nature 392:190.
Gene ID: 999 View all products for this Gene ID 12550 View all products for this Gene ID
UniProt: View information about CD324 on UniProt.org

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