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Purified anti-mouse LPAM-1 (Integrin α4&beta
产品名称:
Purified anti-mouse LPAM-1 (Integrin α4&beta
产品类别:
抗体
产品编号:
120602
产品应用:
120602
[价格]
规格 价格 库存
500µg ¥ 3612 1

产品详情

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
TK1 cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
Block, IP, IHC-F - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 ?g per 106 cells in 100 ?l. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: in vitro and in vivo blocking of cell adhesion1-4, in vitro induction of TK1 cell aggregation1, immunoprecipitation4, and immunohistochemical staining of frozen sections5. It has been reported that the DATK32 mAb is able to block a4?7 mediated lymphocyte adhesion to VCAM-1, MAdCAM-1, and fibronectin in vitro and in vivo.?

Application References

(PubMed link indicates BioLegend citation)
  1. Andrew DP, et al. 1994. J. Immunol. 153:3847. (Block)
  2. Berlin C, et al. 1993. Cell 74:185. (Block)
  3. Berlin-Rufenach C, et al. 1999. J. Exp. Med. 189:1467. (Block)
  4. Rivera-Nieves J, et al. 2005. J. Immunol. 174:2343. (Block, IP)
  5. Bogetto L, et al. 2000. Blood 95:2397. (IHC)
Product Citations
  1. Toubai T, et al. 2017. Blood Adv. 1.095138889. PubMed
RRID
AB_493264 (BioLegend Cat. No. 120602)

Antigen Details

Structure
Integrin family, Ig superfamily, 150 kD/130 kD heterodimer
Distribution

Majority of peripheral lymphocytes, subsets of thymocytes and bone marrow cells

Function
Lymphocyte adhesion
Ligand/Receptor
VCAM-1 (CD106), MAdCAM-1, fibronectin
Cell Type
Lymphocytes, Thymocytes
Biology Area
Cell Biology, Immunology, Neuroscience, Synaptic Biology
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Andrew DP, et al. 1994. J. Immunol. 153:3847.
2. Berlin C, et al. 1994. Cell 74:185.
3. Gurish MF, et al. 2001 J. Exp. Med. 194:1243.
4. Hamann A, et al. 1994. J. Immunol. 152:3282.

Gene ID
311144 View all products for this Gene ID 25713 View all products for this Gene ID
UniProt
View information about LPAM-1 on UniProt.org

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