Ultra-LEAF™ Purified anti-mouse IL-9 Antibod-上海雅吉生物科技有限公司

规格	价格	库存
100µg	￥ 3780	1

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Armenian Hamster

Immunogen

Baculovirus-expressed, recombinant mouse IL-9

Formulation

0.2 ?m filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is < 0.01 EU/?g of the protein (< 0.001 ng/?g of the protein) as determined by the LAL test.

Preparation

The Ultra-LEAF? (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.

Concentration

The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF? solution contains no preservative; handle under aseptic conditions.

Application

Neut - Quality tested
WB - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by ELISA assay. For neutralization of one unit/ml of IL-9, a concentration of 1 - 10 ?g/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

ELISA Detection^1,2: The biotinylated D9302C12 antibody is useful as a detection antibody for a sandwich ELISA assay, when used in conjunction with Purified D8402E8 antibody (Cat. No. 504702) as the capture antibody.
Neutralization^2-5:The Ultra-LEAF? Purified antibody (Endotoxin < 0.01 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for neutralization of mouse IL-9 bioactivity (Cat. No. 504807 and 504808).

Additional reported applications (for the relevant formats) include: Western blotting.

Application References (PubMed link indicates BioLegend citation)

Abrams, J. 1995. Curr. Prot. Immunol. John Wiley and Sons, New York. Unit 6.20.
Renauld, J., et al. 1995. J. Leuk. Biol. 57:353.
Sitkauskiene, B., et al. 2005. Respir Res. 6:33.
Jones, T.G, et al. 2009. J Immunol. 183:5251-60. (Neut) PubMed
Jones, T.G, et al. 2010. J. Immunol. 185:1804-11. (Neut) PubMed

RRID

AB_2832802 (BioLegend Cat. No. 504807) AB_2832803 (BioLegend Cat. No. 504808)

Antigen Details

Structure: Cytokine; 30-40 kD (Mammalian)
Bioactivity: Potentiates production of IgG, IgM, and IgE by IL-4-induced B lymphocytes; regulates granzyme family proteases; enhances proliferation of bone marrow mast cells with IL-3; production of IL-6 by mast cells
Cell Sources: IL-2 activated T_H2 lymphocytes
Cell Targets: T lymphocytes, erythroid precursors
Receptors: IL-9R
Biology Area: Immunology
Molecular Family: Cytokines/Chemokines
Antigen References: 1. Fitzgerald, K., et al. Eds. 2001. The Cytokine FactsBook. Academic Press, San Diego.
2. Quesniaux, V. 1992. Research Immunology 143:385.
3. Renauld, J., et al. 1993. Adv. Immunol. 54:79.
4. Yang, Y. 1992. Leuk. Lymphoma 8:441.
Regulation: Upregulated by IL-1 after TCR activation, TNF-α; downregulated by antibodies to IL-2
Gene ID: 16198 View all products for this Gene ID
UniProt: View information about IL-9 on UniProt.org

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