HRP anti-Alpha-II Spectrin Antibody -上海雅吉生物科技有限公司

规格	价格	库存
25µg	￥ 2058	1

Product Details

Verified Reactivity

Human, Mouse, Rat, Drosophila

Antibody Type

Monoclonal

Host Species

Mouse

Formulation

This antibody is provided in 50% glycerol in aqueous buffered solutions with preservatives.

Preparation

The antibody was purified by affinity chromatography and conjugated with HRP under optimal conditions.

Concentration

0.5 mg/ml

Storage & Handling

Upon receipt, the antibody solution should be stored undiluted at -20°C, and protected from prolonged exposure to light.

Application

WB - Quality tested
IHC-P - Verified

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1.0 - 2.5 ?g per ml. For formalin-fixed paraffin-embedded immunohistochemical staining, a concentration range of 1.0 - 5.0 ?g/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Clone D8B7 is a specific marker for human, rat and mouse alpha II spectrin or fodrin. In cerebellar cell cultures, it labels axons and periphery of cell bodies consistent with localization of fodrin (reviewed in Bennett, 1993; Goodman, 1995 & De Matteis, 2000)^{4, 6, 8}. It does not cross-react with alpha I (erythroid) spectrin SH3 domain.

This antibody was developed against a recombinant alpha II spectrin (non-erythroid spectrin or fodrin) SH3 domain. It was subsequently purified via ammonium sulfate precipitation.

Reactivity to Drosophila was only verified with the purified format.

Application References (PubMed link indicates BioLegend citation)

Konieczny P, et al. 2008. J Cell Biol. 181:667-81
Xu J, et al. 2001. Brain Res. 898:171-7 (WB, IF)
Xu J, et al. 2000. J Cell Science. 113:3805-14
De Matteis MA and Morrow JS. 2000. J. Cell Science. 113:2331-43
Swanson JA and Watts C. 1995. Trends Cell Biol. 5:424-427
Goodman SR, et al. 1995. Brain Res Bull. 36:593-606
Lamaze C and Schimid SL. 1995. Curr Opin Cell Biol. 7:573-580
Bennett V and Gilligan DM. 1993. Annu Rev Cell Biol. 9:27-66
Huang C, et al. 2017. J Neurosci. 37:11323-11334 PubMed

RRID

AB_2810702 (BioLegend Cat. No. 803207) AB_2810703 (BioLegend Cat. No. 803208)

Antigen Details

Structure

Alpha-II Spectrin is a 2472 amino acid protein with an expected molecular mass of 285 kD. A cleavage product of ~ 150 kD is observed in tissue lysates (1) (2).

Biology Area

Cell Biology, Cell Motility/Cytoskeleton/Structure, Neuroscience, Neuroscience Cell Markers

Molecular Family

Presynaptic proteins, Scaffold Proteins

Antigen References