Anti-Alpha-II Spectrin Antibody (Previously Covan-上海雅吉生物科技有限公司

规格	价格	库存
200µL	￥ 8134	1

Product Details

Verified Reactivity

Human, Mouse, Rat, Drosophila

Antibody Type

Monoclonal

Host Species

Mouse

Preparation

Ascites

Storage & Handling

Store at -20°C or below. Upon initial thawing, apportion into working aliquots and store at -20°C or below. Avoid repeated freeze-thaw cycles to prevent denaturing the antibody. Do not store in frost-free freezers.

Application

WB - Quality tested
IHC-P, ICC - Verified

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1:2000 - 1:4000 dilution.?For immunohistochemistry on formalin-fixed paraffin-embedded tissue, a dilution of 1:1000 is suggested.?For immunocytochemistry, a dilution of 1:1000 is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Clone D8B7 is a specific marker for human, rat and mouse alpha II spectrin or fodrin. In cerebellar cell cultures, it labels axons and periphery of cell bodies consistent with localization of fodrin (reviewed in Bennett, 1993; Goodman, 1995 & De Matteis, 2000)^{4, 6, 8}. It does not cross-react with alpha I (erythroid) spectrin SH3 domain.

This antibody was developed against a recombinant alpha II spectrin (non-erythroid spectrin or fodrin) SH3 domain. It was subsequently purified via ammonium sulfate precipitation.

Reactivity to Drosophila was only verified with the purified format.

Application References (PubMed link indicates BioLegend citation)

Konieczny P, et al. 2008. J Cell Biol. 181:667-81
Xu J, et al. 2001. Brain Res. 898:171-7 (WB, IF)
Xu J, et al. 2000. J Cell Science. 113:3805-14
De Matteis MA and Morrow JS. 2000. J. Cell Science. 113:2331-43
Swanson JA and Watts C. 1995. Trends Cell Biol. 5:424-427
Goodman SR, et al. 1995. Brain Res Bull. 36:593-606
Lamaze C and Schimid SL. 1995. Curr Opin Cell Biol. 7:573-580
Bennett V and Gilligan DM. 1993. Annu Rev Cell Biol. 9:27-66
Huang C, et al. 2017. J Neurosci. 37:11323-11334 PubMed

Product Citations

Wang G et al. 2019. eLife. 8 pii: e38730. PubMed
Miazek A, et al. 2021. Scientific Reports. 11(1):7312. PubMed
Torii T, et al. 2020. The Journal of Cell Biology. 219(2):e201907048. PubMed
Huang CY et al. 2017. The Journal of Neuroscience. 37(47):11323-11334 . PubMed
Zhou R, et al. 2022. Nat Commun. 13:3196. PubMed
Lubbers ER, et al. 2019. J Biol Chem. 294:9576. PubMed
Hamdan H, et al. 2020. Nat Commun. 0.527777778. PubMed
Huang CY et al. 2017. The Journal of Neuroscience. 37(47):11311-11322 . PubMed
Liu Y, et al. 2019. Sci Adv. 5:eaav7803. PubMed
Cousin MA, et al. 2021. Nat Genet. 53:1006. PubMed

RRID

AB_2564660 (BioLegend Cat. No. 803201)

Antigen Details

Structure

Alpha-II Spectrin is a 2472 amino acid protein with an expected molecular mass of 285 kD. A cleavage product of ~ 150 kD is observed in tissue lysates (1) (2).

Biology Area

Cell Biology, Cell Motility/Cytoskeleton/Structure, Neuroscience, Neuroscience Cell Markers

Molecular Family

Presynaptic proteins, Scaffold Proteins

Antigen References