Alexa Fluor? 647 anti-Alpha-II Spectrin Antibody -上海雅吉生物科技有限公司

规格	价格	库存
25µg	￥ 2226	1

Product Details

Verified Reactivity

Human, Mouse, Rat, Drosophila

Antibody Type

Monoclonal

Host Species

Mouse

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide

Preparation

The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 647 under optimal conditions.

Concentration

0.5 mg/mL

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

IHC-P - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 1.0 - 10 ?g/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor? 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.

Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.

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Excitation Laser

Red Laser (633 nm)

Application Notes

Clone D8B7 is a specific marker for human, rat and mouse alpha II spectrin or fodrin. In cerebellar cell cultures, it labels axons and periphery of cell bodies consistent with localization of fodrin (reviewed in Bennett, 1993; Goodman, 1995 & De Matteis, 2000)^{4, 6, 8}. It does not cross-react with alpha I (erythroid) spectrin SH3 domain.

This antibody was developed against a recombinant alpha II spectrin (non-erythroid spectrin or fodrin) SH3 domain. It was subsequently purified via ammonium sulfate precipitation.

Reactivity to Drosophila was only verified with the purified format.

Application References (PubMed link indicates BioLegend citation)

Konieczny P, et al. 2008. J Cell Biol. 181:667-81
Xu J, et al. 2001. Brain Res. 898:171-7 (WB, IF)
Xu J, et al. 2000. J Cell Science. 113:3805-14
De Matteis MA and Morrow JS. 2000. J. Cell Science. 113:2331-43
Swanson JA and Watts C. 1995. Trends Cell Biol. 5:424-427
Goodman SR, et al. 1995. Brain Res Bull. 36:593-606
Lamaze C and Schimid SL. 1995. Curr Opin Cell Biol. 7:573-580
Bennett V and Gilligan DM. 1993. Annu Rev Cell Biol. 9:27-66
Huang C, et al. 2017. J Neurosci. 37:11323-11334 PubMed

RRID

AB_2820121 (BioLegend Cat. No. 803211) AB_2820122 (BioLegend Cat. No. 803212)

Antigen Details

Structure

Alpha-II Spectrin is a 2472 amino acid protein with an expected molecular mass of 285 kD. A cleavage product of ~ 150 kD is observed in tissue lysates (1) (2).

Biology Area

Cell Biology, Cell Motility/Cytoskeleton/Structure, Neuroscience, Neuroscience Cell Markers

Molecular Family

Presynaptic proteins, Scaffold Proteins

Antigen References