Ultra-LEAF™ Purified anti-mouse CD126 (IL-6R-上海雅吉生物科技有限公司

规格	价格	库存
100µg	￥ 2548	1

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Rat

Immunogen

OKT4 hybridoma cells

Formulation

0.2 ?m filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is <0.01 EU/?g of the protein (<0.001 ng/?g of the protein) as determined by the LAL test.

Preparation

The Ultra-LEAF? (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.

Concentration

The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF? solution contains no preservative; handle under aseptic conditions.

Application

FC - Quality tested
Block, IP, Neut - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation^1,2,5,?in vivo receptor blocking^1-2,4-6,8, and in vitro neutralization⁷. For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate (Cat. No. 115806) or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated antibody (Cat. No. 115804) or biotinylated anti-rat IgG second step (Cat. No. 405402), followed by SAv-PE (Cat. No. 405204)). The LEAF? purified antibody (Endotoxin <0.1 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. No. 115808).

Please note, this clone had previously reported reactivity to rat, however upon further testing it has been determined to not react with rat.

Application References (PubMed link indicates BioLegend citation)

Coulie PG, et al. 1990. Curr. Top. Microbiol. Immunol. 166:43. (Block, IP)
Vink A, et al. 1990. J. Exp. Med. 172:997. (Block, IP)
Sun R, et al. 2004. J. Immunol. 172:5648. (FC)
McKinney WJ, et al. 1998. Am. J. Respir. Cell Mol. Biol. 18:696. (Block)
Weigert C, et al. 2006. J. Biol. Chem. 281:7060. (Block, IP)
Koizumi N, et al. 2007. J. Immunol. 178:1767. (Block)
Walker F, et al. 2008. Blood 111:3978. (Neut) PubMed
Yadav M, et al. 2008. J. Immunol. 180:2772. (Block) PubMed
Sintes J, et al. 2010. J. Leukoc. Biol. 88:687. PubMed

RRID

AB_2810349 (BioLegend Cat. No. 115815) AB_2810350 (BioLegend Cat. No. 115816)

Antigen Details

Structure: Ig superfamily, associates with IL-6Rβ chain (CD130, gp130), 80 kD
Distribution: Activated T cells and B cells, monocytes, hepatocytes, plasma cells
Function: Stimulates T cells, B cells, hematopoietic precursor proliferation and differentiation
Ligand/Receptor: IL-6
Cell Type: B cells, Mesenchymal Stem Cells, Monocytes, Plasma cells, T cells, Tregs
Biology Area: Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience, Signal Transduction
Molecular Family: CD Molecules, Cytokine/Chemokine Receptors
Antigen References: 1. Taga T, et al. 1997. Annu. Rev. Immunol. 15:797.
2. Fitzgerald K, et al. 2001. The Cytokine FactsBook. Academic Press London.
3. Boulanger MJ, et al. 2003. Science 300:2101.
Gene ID: 16194 View all products for this Gene ID
UniProt: View information about CD126 on UniProt.org

上海细胞库

原代细胞

细胞培养

细胞服务

酶联试剂盒

生化试剂盒

PCR试剂盒

抗体蛋白

试剂耗材

首页 > 抗体蛋白 > 抗体

产品详情

Product Details

Antigen Details

联系我们