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Purified anti-mouse Ly-6A/E (Sca-1) Antibody
产品名称：
Purified anti-mouse Ly-6A/E (Sca-1) Antibody
产品类别：
抗体
产品编号：
108101
产品应用：
108101
[价格]
规格 价格 库存
50µg ￥ 826 1

产品详情

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
IL-2-dependent mouse T-cell line (CTL-L)
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
WB, IP, IHC - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 ?g per 106 cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

The D7 antibody has been reported to induce T cell activation and inhibit TCR-induced IL-2 production. Additional reported applications (for the relevant formats) include: Western blotting1,2, immunoprecipitation1, in vitro lymphocyte activation3-6, induction of redirected lysis7, induction of T cell inhibitory signalling8, immunofluorescence9, and immunohistochemical staining of acetone-fixed frozen sections13 and Bouin-fixed,?paraffin-embedded samples9.

The two Sca-1 recognizing clones?D7?and E13-161.7 have been shown to bind distinct epitopes due to the inability of D7 to block the binding of E13-161.7.14?

Application References

(PubMed link indicates BioLegend citation)
  1. Ortega G, et al. 1986. J. Immunol. 137:3240. (WB, IP)
  2. Palfree RGE, et al. 1986. Immunogenetics 23:197. (WB)
  3. Codias EK, et al. 1990. J. Immunol. 144:2197.
  4. Malek TR, et al. 1986. J. Exp. Med. 164:709.
  5. Codias EK, et al. 1990. J. Immunol. 145:1407.
  6. Ivanov V, et al. 1994. J. Immunol. 153:2394.
  7. Karlhofer FM, et al. 1991. J. Immunol. 146:3662.
  8. Fleming T, et al. 1994. J. Immunol. 153:1955.
  9. van Bragt MPA, et al. 2005. Biol. Reprod. 73:634. (IF, IHC)
  10. Umland O, et al. 2007. J. Immunol. 178:4147.
  11. Cridland SO, et al. 2009. Blood Cell. Mol. Dis. 45:149. (FC) PubMed
  12. Pronk CJ, et al. 2011. J. Exp Med. PubMed
  13. English A, et al. 2000. J. Immunol. 165:3763. (IHC)
  14. Bamezai A and Rock KL. 1995. Proc. Natl. Acad. Sci. USA 92:4294.
  15. Wiesner DL, et al. 2015. PLoS Pathog. 11:1004701. PubMed
Product Citations
  1. Khan KA, et al. 2020. NPJ Breast Cancer. 6:29. PubMed
  2. Weng L, et al. 2016. Sci Rep. 6:38632. PubMed
  3. Reeves PM, et al. 2017. Oncotarget. 8:94040. PubMed
  4. Chen S, et al. 2022. Cancer Discov. :. PubMed
  5. Hu X, et al. 2016. Nat Commun. 7:13095. PubMed
  6. Paulo E, et al. 2018. Sci Rep. 8:11001. PubMed
  7. Casey AE, et al. 2018. J Cell Biol. 217:2951. PubMed
  8. Inoue D, et al. 2021. Nat Genet. 53:707. PubMed
  9. Yoshimi A, et al. 2019. Nature. 574:273. PubMed
  10. Suzuki Y, et al. 2021. FEBS Open Bio. 11:2619. PubMed
  11. Khan KA, et al. 2020. NPJ Breast Cancer. 6:29. PubMed
  12. Cardoso F, et al. 2021. Nature. 597:410. PubMed
  13. Zhong X, et al. 2020. Proc Natl Acad Sci U S A. 8563:117. PubMed
  14. Park JW, et al. 2022. Cancer Med. 11:1925. PubMed
  15. Blriot C, et al. 2021. Immunity. :. PubMed
  16. Gubin MM, et al. 2018. Cell. 175:1014. PubMed
  17. Karmaus PWF, et al. 2019. Nature. 565:101. PubMed
  18. Pronk C, et al. 2011. J Exp Med. 208:1563. PubMed
  19. Krotenberg Garcia A, et al. 2021. Cell Reports. 36(1):109307. PubMed
  20. Oherle K, et al. 2020. Immunity. 275:52. PubMed
  21. Link CWM, et al. 2020. Front Immunol. 11:596772. PubMed
  22. Iida Y, et al. 2020. J Immunother Cancer. 8:. PubMed
  23. Samala R, et al. 2013. J Proteomics. 75:5304. PubMed
  24. Burton OT, et al. 2018. Clin Exp Allergy. 48:288. PubMed
  25. Silva J, et al. 2022. Nat Commun. 13:4492. PubMed
RRID
AB_313338 (BioLegend Cat. No. 108101) AB_313339 (BioLegend Cat. No. 108102)

Antigen Details

Structure
Ly-6 multigene family, 18 kD
Distribution

Hematopoietic stem cells, activated T cells and B cells, subset of resting B cells and T cells

Function
Regulates B and T cell responses
Cell Type
B cells, Hematopoietic stem and progenitors, Mesenchymal Stem Cells, T cells
Biology Area
Immunology, Stem Cells
Antigen References

1. Rock KL, et al. 1989. Immunol. Rev. 111:195.
2. Morrison SJ, et al. 1994. Immunity 1:661.
3. Spangrude GJ, et al. 1988. J. Immunol. 141:3697.
4. Malek T, et al. 1986. J. Exp. Med. 164:709.

Gene ID
110454 View all products for this Gene ID
UniProt
View information about Ly-6A/E on UniProt.org

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