Purified anti-mouse Ly-6A/E (Sca-1) (Maxpar® R-上海雅吉生物科技有限公司

规格	价格	库存
100µg	￥ 1568	1

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Rat

Immunogen

IL-2-dependent mouse T-cell line (CTL-L)

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA.

Preparation

The antibody was purified by affinity chromatography.

Concentration

1.0 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C.

Application

FC - Quality tested
CyTOF? - Verified

Recommended Usage

This product is suitable for use with the?Maxpar? Metal Labeling Kits.?For metal labeling using Maxpar? Ready antibodies,?proceed directly to the step to Partially Reduce the Antibody by adding 100 ?l of Maxpar? Ready antibody to 100 ?l of 4 mM TCEP-R in a 50 kDa filter and continue with the protocol. Always refer to the latest version of Maxpar? User Guide when conjugating Maxpar? Ready antibodies.

Application Notes

The D7 antibody has been reported to induce T cell activation and inhibit TCR-induced IL-2 production. Additional reported applications (for the relevant formats) include: Western blotting^1,2, immunoprecipitation¹, in vitro lymphocyte activation^3-6, induction of redirected lysis⁷, induction of T cell inhibitory signalling⁸, immunofluorescence⁹, and immunohistochemical staining of acetone-fixed frozen sections¹³ and Bouin-fixed,?paraffin-embedded samples⁹.

The two Sca-1 recognizing clones?D7?and E13-161.7 have been shown to bind distinct epitopes due to the inability of D7 to block the binding of E13-161.7.¹⁴?

Additional Product Notes

Maxpar? is a registered trademark of Standard BioTools Inc.

Application References (PubMed link indicates BioLegend citation)

Ortega G, et al. 1986. J. Immunol. 137:3240. (WB, IP)
Palfree RGE, et al. 1986. Immunogenetics 23:197. (WB)
Codias EK, et al. 1990. J. Immunol. 144:2197.
Malek TR, et al. 1986. J. Exp. Med. 164:709.
Codias EK, et al. 1990. J. Immunol. 145:1407.
Ivanov V, et al. 1994. J. Immunol. 153:2394.
Karlhofer FM, et al. 1991. J. Immunol. 146:3662.
Fleming T, et al. 1994. J. Immunol. 153:1955.
van Bragt MPA, et al. 2005. Biol. Reprod. 73:634. (IF, IHC)
Umland O, et al. 2007. J. Immunol. 178:4147.
Cridland SO, et al. 2009. Blood Cell. Mol. Dis. 45:149. (FC) PubMed
Pronk CJ, et al. 2011. J. Exp Med. PubMed
English A, et al. 2000. J. Immunol. 165:3763. (IHC)
Bamezai A and Rock KL. 1995. Proc. Natl. Acad. Sci. USA 92:4294.
Wiesner DL, et al. 2015. PLoS Pathog. 11:1004701. PubMed

Product Citations

Wei SC, et al. 2019. Immunity. 50:1084. PubMed
Barvalia M, et al. 2022. Methods Mol Biol. 2508:147. PubMed
McClellan BL, et al. 2022. STAR Protoc. 3:101357. PubMed

RRID

AB_2563782 (BioLegend Cat. No. 108135)

Antigen Details

Structure: Ly-6 multigene family, 18 kD
Distribution: Hematopoietic stem cells, activated T cells and B cells, subset of resting B cells and T cells
Function: Regulates B and T cell responses
Cell Type: B cells, Hematopoietic stem and progenitors, Mesenchymal Stem Cells, T cells
Biology Area: Immunology, Stem Cells
Antigen References: 1. Rock KL, et al. 1989. Immunol. Rev. 111:195.
2. Morrison SJ, et al. 1994. Immunity 1:661.
3. Spangrude GJ, et al. 1988. J. Immunol. 141:3697.
4. Malek T, et al. 1986. J. Exp. Med. 164:709.
Gene ID: 110454 View all products for this Gene ID
UniProt: View information about Ly-6A/E on UniProt.org

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