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首页 > 抗体蛋白 > 抗体
PerCP/Cyanine5.5 anti-mouse Ly-6A/E (Sca-1) Antibo
产品名称：
PerCP/Cyanine5.5 anti-mouse Ly-6A/E (Sca-1) Antibo
产品类别：
抗体
产品编号：
108123
产品应用：
108123
[价格]
规格 价格 库存
25µg ￥ 1652 1

产品详情

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
IL-2-dependent mouse T-cell line (CTL-L)
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is = 0.25 ?g per 106 cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Application Notes

The D7 antibody has been reported to induce T cell activation and inhibit TCR-induced IL-2 production. Additional reported applications (for the relevant formats) include: Western blotting1,2, immunoprecipitation1, in vitro lymphocyte activation3-6, induction of redirected lysis7, induction of T cell inhibitory signalling8, immunofluorescence9, and immunohistochemical staining of acetone-fixed frozen sections13 and Bouin-fixed,?paraffin-embedded samples9.

The two Sca-1 recognizing clones?D7?and E13-161.7 have been shown to bind distinct epitopes due to the inability of D7 to block the binding of E13-161.7.14?

Additional Product Notes

BioLegend is in the process of converting the name PerCP/Cy5.5 to PerCP/Cyanine5.5. The dye molecule remains the same, so you should expect the same quality and performance from our PerCP/Cyanine5.5 products. Contact Technical Service if you have any questions.

Application References

(PubMed link indicates BioLegend citation)
  1. Ortega G, et al. 1986. J. Immunol. 137:3240. (WB, IP)
  2. Palfree RGE, et al. 1986. Immunogenetics 23:197. (WB)
  3. Codias EK, et al. 1990. J. Immunol. 144:2197.
  4. Malek TR, et al. 1986. J. Exp. Med. 164:709.
  5. Codias EK, et al. 1990. J. Immunol. 145:1407.
  6. Ivanov V, et al. 1994. J. Immunol. 153:2394.
  7. Karlhofer FM, et al. 1991. J. Immunol. 146:3662.
  8. Fleming T, et al. 1994. J. Immunol. 153:1955.
  9. van Bragt MPA, et al. 2005. Biol. Reprod. 73:634. (IF, IHC)
  10. Umland O, et al. 2007. J. Immunol. 178:4147.
  11. Cridland SO, et al. 2009. Blood Cell. Mol. Dis. 45:149. (FC) PubMed
  12. Pronk CJ, et al. 2011. J. Exp Med. PubMed
  13. English A, et al. 2000. J. Immunol. 165:3763. (IHC)
  14. Bamezai A and Rock KL. 1995. Proc. Natl. Acad. Sci. USA 92:4294.
  15. Wiesner DL, et al. 2015. PLoS Pathog. 11:1004701. PubMed
Product Citations
  1. Tober J, et al. 2020. The Journal of Experimental Medicine. 215(2):645-659. PubMed
  2. Riether C, et al. 2021. Cell Reports. 34(4):108663. PubMed
  3. Delás MJ, et al. 2019. Cell Rep. 27:719. PubMed
  4. Helm M, et al. 2022. Life (Basel). 12:. PubMed
  5. Paiva RS, et al. 2021. Eur J Immunol. 51:1968. PubMed
  6. Paiva RA, et al. 2021. Cell Reports. 35(2):108967. PubMed
  7. Reddy A, et al. 2020. Cell. 183:62. PubMed
  8. Taube C, et al. 2011. PLoS One. 6:e21799. PubMed
  9. Barman PK, et al. 2019. J Immunol. 202:2720. PubMed
  10. Li Y, et al. 2020. Cell Stem Cell. 27(5):732-747.e7. PubMed
  11. Liu W, et al. 2019. Epigenetics. 1.192361111. PubMed
  12. Chen R, et al. 2021. Cell Reports. 34(7):108751. PubMed
  13. Zheng X, et al. 2015. Cell Rep. 13:1505-1518. PubMed
  14. de Laval B, et al. 2020. Cell Stem Cell. 26(5):657-674. PubMed
  15. Duarte D, et al. 2018. Cell Stem Cell. 22:64. PubMed
  16. Nenasheva T, et al. 2017. PLoS One. 12(6):e0178983. PubMed
  17. Baja?a S, et al. 2022. iScience. 25:103732. PubMed
  18. van den Berk P, et al. 2020. Cell Rep. 33:108533. PubMed
  19. DeVilbiss AW, et al. 2021. eLife. 10:00. PubMed
  20. Gago-Lopez N, et al. 2019. EMBO Mol Med. 11:e10697. PubMed
  21. Pascual R, et al. 2020. Sci Adv. 6:eaax3868. PubMed
  22. Li K, et al. 2020. Nat Commun. 0.795138889. PubMed
  23. Gu Z, et al. 2021. Nat Genet. 53:672. PubMed
  24. Cohen I et al. 2018. Cell stem cell. 22(5):726-739 . PubMed
RRID
AB_893619 (BioLegend Cat. No. 108123) AB_893615 (BioLegend Cat. No. 108124)

Antigen Details

Structure
Ly-6 multigene family, 18 kD
Distribution

Hematopoietic stem cells, activated T cells and B cells, subset of resting B cells and T cells

Function
Regulates B and T cell responses
Cell Type
B cells, Hematopoietic stem and progenitors, Mesenchymal Stem Cells, T cells
Biology Area
Immunology, Stem Cells
Antigen References

1. Rock KL, et al. 1989. Immunol. Rev. 111:195.
2. Morrison SJ, et al. 1994. Immunity 1:661.
3. Spangrude GJ, et al. 1988. J. Immunol. 141:3697.
4. Malek T, et al. 1986. J. Exp. Med. 164:709.

Gene ID
110454 View all products for this Gene ID
UniProt
View information about Ly-6A/E on UniProt.org

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