热线电话: 021-34661275
产品分类/PRODUCT CLASSIFICATION

上海细胞库

人源细胞系| 稳转细胞系| 基因敲除株| 基因点突变细胞株| 基因过表达细胞株| 重组细胞系| 猪的细胞系| 马细胞系| 兔的细胞系| 犬的细胞系| 山羊的细胞系| 鱼的细胞系| 猴的细胞系| 仓鼠的细胞系| 狗的细胞系| 牛的细胞| 大鼠细胞系| 小鼠细胞系| 其他细胞系|

原代细胞

原代细胞| 永生化细胞|

细胞培养

血清| 无血清冻存| 细胞培养试剂盒| 普通培养基| 完全培养基| 细胞污染预防| 细胞污染清除| 细胞培养辅助试剂|

细胞服务

STR鉴定服务| ATCC代购服务|

酶联试剂盒

人ELISA试剂盒| 大鼠ELISA试剂盒| 小鼠ELISA试剂盒| 兔ELISA试剂盒| 猪ELISA试剂盒| 鱼ELISA试剂盒| 牛ELISA试剂盒| 犬ELISA试剂盒| 鸭ELISA试剂盒| 豚鼠ELISA试剂盒| 鸡ELISA试剂盒| 绵羊ELISA试剂盒| 山羊ELISA试剂盒| 仓鼠ELISA试剂盒| 植物ELISA试剂盒| 药残留类试剂盒| 其他ELISA试剂盒|

生化试剂盒

生化试剂盒| 微量法检测盒| 分光法检测盒| 胶体金检测盒|

PCR试剂盒

荧光探针PCR试剂盒| 染料荧光PCR试剂盒| 核酸扩增/纯化| 普通PCR检测试剂盒| 质粒菌种感受态|

抗体蛋白

蛋白多肽| 抗体| 免疫组化| 免疫球蛋白|

试剂耗材

标准试液| 科研试剂| 缓 冲 液| 实验耗材| 高效液相色谱对照品|

首页 > 抗体蛋白 > 抗体
FITC anti-mouse Ly-6A/E (Sca-1) Antibody
产品名称：
FITC anti-mouse Ly-6A/E (Sca-1) Antibody
产品类别：
抗体
产品编号：
108105
产品应用：
108105
[价格]
规格 价格 库存
50µg ￥ 994 1

产品详情

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
IL-2-dependent mouse T-cell line (CTL-L)
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Application Notes

The D7 antibody has been reported to induce T cell activation and inhibit TCR-induced IL-2 production. Additional reported applications (for the relevant formats) include: Western blotting1,2, immunoprecipitation1, in vitro lymphocyte activation3-6, induction of redirected lysis7, induction of T cell inhibitory signalling8, immunofluorescence9, and immunohistochemical staining of acetone-fixed frozen sections13 and Bouin-fixed,?paraffin-embedded samples9.

The two Sca-1 recognizing clones?D7?and E13-161.7 have been shown to bind distinct epitopes due to the inability of D7 to block the binding of E13-161.7.14?

Application References

(PubMed link indicates BioLegend citation)
  1. Ortega G, et al. 1986. J. Immunol. 137:3240. (WB, IP)
  2. Palfree RGE, et al. 1986. Immunogenetics 23:197. (WB)
  3. Codias EK, et al. 1990. J. Immunol. 144:2197.
  4. Malek TR, et al. 1986. J. Exp. Med. 164:709.
  5. Codias EK, et al. 1990. J. Immunol. 145:1407.
  6. Ivanov V, et al. 1994. J. Immunol. 153:2394.
  7. Karlhofer FM, et al. 1991. J. Immunol. 146:3662.
  8. Fleming T, et al. 1994. J. Immunol. 153:1955.
  9. van Bragt MPA, et al. 2005. Biol. Reprod. 73:634. (IF, IHC)
  10. Umland O, et al. 2007. J. Immunol. 178:4147.
  11. Cridland SO, et al. 2009. Blood Cell. Mol. Dis. 45:149. (FC) PubMed
  12. Pronk CJ, et al. 2011. J. Exp Med. PubMed
  13. English A, et al. 2000. J. Immunol. 165:3763. (IHC)
  14. Bamezai A and Rock KL. 1995. Proc. Natl. Acad. Sci. USA 92:4294.
  15. Wiesner DL, et al. 2015. PLoS Pathog. 11:1004701. PubMed
Product Citations
  1. Ludwik KA, et al. 2020. Cell Reports. 32(3):107931. PubMed
  2. Johnson KD, et al. 2022. Blood Adv. 6:1464. PubMed
  3. Yamaguchi A, et al. 2021. Genes Cells. 26:782. PubMed
  4. Bowers E, et al. 2018. Nat Med. 24:95. PubMed
  5. Cha S, et al. 2021. Int J Mol Sci. 22:. PubMed
  6. Mittal S, et al. 2016. Stem Cell Reports. 7:583-590. PubMed
  7. Hughes RM, et al. 2020. Cancer Research. 79(14):3636-3650.. PubMed
  8. Dey A et al. 2019. The EMBO journal. 38(7) pii: e100293. PubMed
  9. Stivala S, et al. 2019. J Clin Invest. 130:1596. PubMed
  10. He X, et al. 2021. Adv Sci (Weinh). 8:e2103023. PubMed
  11. Wu L, et al. 2022. Theranostics. 12:842. PubMed
  12. Yang SH, et al. 2017. Front Immunol. 8:1192. PubMed
  13. Lima-Junior DS, et al. 2021. Cell. . PubMed
  14. Zhang H, et al. 2019. Mol Cell. 76:110. PubMed
  15. Kim JH, et al. 2021. J Cachexia Sarcopenia Muscle. 12:177. PubMed
  16. Zhou J, et al. 2019. Nat Commun. 10:2427. PubMed
  17. Kim J, et al. 2016. Nat Cell Biol. 18:930-940. PubMed
  18. Zheng X, et al. 2015. Cell Rep. 13:1505-1518. PubMed
  19. Kanemaru K, et al. 2015. PLoS One. 10: 0144436. PubMed
  20. Ghosh D, et al. 2016. J Immunol. 197: 1788 - 1800. PubMed
  21. Deng P, et al. 2021. Cell Stem Cell. 28(6):1057-1073.e7. PubMed
  22. Baja?a S, et al. 2022. iScience. 25:103732. PubMed
  23. Koide S, et al. 2022. iScience. 25:103603. PubMed
  24. Bao Z, et al. 2020. Ann Transplant. 25:e921287. PubMed
  25. Wilkinson AC, et al. 2019. Nature. 571:117. PubMed
  26. Woods B, et al. 2019. Clin Cancer Res. 25:5901. PubMed
  27. Halvarsson C, et al. 2019. Antioxid Redox Signal. 31:211. PubMed
  28. Dourcy M, et al. 2020. Mucosal Immunol. 13:799. PubMed
  29. Radulovic V, et al. 2020. Cell Reports. 27(10):2826-2836.e5.. PubMed
RRID
AB_313342 (BioLegend Cat. No. 108105) AB_313343 (BioLegend Cat. No. 108106)

Antigen Details

Structure
Ly-6 multigene family, 18 kD
Distribution

Hematopoietic stem cells, activated T cells and B cells, subset of resting B cells and T cells

Function
Regulates B and T cell responses
Cell Type
B cells, Hematopoietic stem and progenitors, Mesenchymal Stem Cells, T cells
Biology Area
Immunology, Stem Cells
Antigen References

1. Rock KL, et al. 1989. Immunol. Rev. 111:195.
2. Morrison SJ, et al. 1994. Immunity 1:661.
3. Spangrude GJ, et al. 1988. J. Immunol. 141:3697.
4. Malek T, et al. 1986. J. Exp. Med. 164:709.

Gene ID
110454 View all products for this Gene ID
UniProt
View information about Ly-6A/E on UniProt.org

联系我们

TEL：021-34661275 　点击拨打热线