热线电话: 021-34661275
产品分类/PRODUCT CLASSIFICATION

上海细胞库

人源细胞系| 稳转细胞系| 基因敲除株| 基因点突变细胞株| 基因过表达细胞株| 重组细胞系| 猪的细胞系| 马细胞系| 兔的细胞系| 犬的细胞系| 山羊的细胞系| 鱼的细胞系| 猴的细胞系| 仓鼠的细胞系| 狗的细胞系| 牛的细胞| 大鼠细胞系| 小鼠细胞系| 其他细胞系|

原代细胞

原代细胞| 永生化细胞|

细胞培养

血清| 无血清冻存| 细胞培养试剂盒| 普通培养基| 完全培养基| 细胞污染预防| 细胞污染清除| 细胞培养辅助试剂|

细胞服务

STR鉴定服务| ATCC代购服务|

酶联试剂盒

人ELISA试剂盒| 大鼠ELISA试剂盒| 小鼠ELISA试剂盒| 兔ELISA试剂盒| 猪ELISA试剂盒| 鱼ELISA试剂盒| 牛ELISA试剂盒| 犬ELISA试剂盒| 鸭ELISA试剂盒| 豚鼠ELISA试剂盒| 鸡ELISA试剂盒| 绵羊ELISA试剂盒| 山羊ELISA试剂盒| 仓鼠ELISA试剂盒| 植物ELISA试剂盒| 药残留类试剂盒| 其他ELISA试剂盒|

生化试剂盒

生化试剂盒| 微量法检测盒| 分光法检测盒| 胶体金检测盒|

PCR试剂盒

荧光探针PCR试剂盒| 染料荧光PCR试剂盒| 核酸扩增/纯化| 普通PCR检测试剂盒| 质粒菌种感受态|

抗体蛋白

蛋白多肽| 抗体| 免疫组化| 免疫球蛋白|

试剂耗材

标准试液| 科研试剂| 缓 冲 液| 实验耗材| 高效液相色谱对照品|

首页 > 抗体蛋白 > 抗体
Brilliant Violet 421™ anti-mouse Ly-6A/E (Sc
产品名称：
Brilliant Violet 421™ anti-mouse Ly-6A/E (Sc
产品类别：
抗体
产品编号：
108127
产品应用：
108127
[价格]
规格 价格 库存
125µL ￥ 2702 1

产品详情

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
IL-2-dependent mouse T-cell line (CTL-L)
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421? under optimal conditions.
Concentration
?g sizes: 0.2 mg/mL
?L sizes: lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining using the ?g size, the suggested use of this reagent is ≤0.25 ?g per million cells in 100 ?l volume. For immunofluorescent staining using the ?l size, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 421? excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421? is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet?.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

The D7 antibody has been reported to induce T cell activation and inhibit TCR-induced IL-2 production. Additional reported applications (for the relevant formats) include: Western blotting1,2, immunoprecipitation1, in vitro lymphocyte activation3-6, induction of redirected lysis7, induction of T cell inhibitory signalling8, immunofluorescence9, and immunohistochemical staining of acetone-fixed frozen sections13 and Bouin-fixed,?paraffin-embedded samples9.

The two Sca-1 recognizing clones?D7?and E13-161.7 have been shown to bind distinct epitopes due to the inability of D7 to block the binding of E13-161.7.14?

Application References

(PubMed link indicates BioLegend citation)
  1. Ortega G, et al. 1986. J. Immunol. 137:3240. (WB, IP)
  2. Palfree RGE, et al. 1986. Immunogenetics 23:197. (WB)
  3. Codias EK, et al. 1990. J. Immunol. 144:2197.
  4. Malek TR, et al. 1986. J. Exp. Med. 164:709.
  5. Codias EK, et al. 1990. J. Immunol. 145:1407.
  6. Ivanov V, et al. 1994. J. Immunol. 153:2394.
  7. Karlhofer FM, et al. 1991. J. Immunol. 146:3662.
  8. Fleming T, et al. 1994. J. Immunol. 153:1955.
  9. van Bragt MPA, et al. 2005. Biol. Reprod. 73:634. (IF, IHC)
  10. Umland O, et al. 2007. J. Immunol. 178:4147.
  11. Cridland SO, et al. 2009. Blood Cell. Mol. Dis. 45:149. (FC) PubMed
  12. Pronk CJ, et al. 2011. J. Exp Med. PubMed
  13. English A, et al. 2000. J. Immunol. 165:3763. (IHC)
  14. Bamezai A and Rock KL. 1995. Proc. Natl. Acad. Sci. USA 92:4294.
  15. Wiesner DL, et al. 2015. PLoS Pathog. 11:1004701. PubMed
Product Citations
  1. Grigsby SM, et al. 2021. Cancers (Basel). 13:. PubMed
  2. Ho YH, et al. 2019. Cell Stem Cell. 25:407. PubMed
  3. Schloss MJ, et al. 2022. Nat Immunol. 23:605. PubMed
  4. Groenen AG, et al. 2022. J Lipid Res. 63:100167. PubMed
  5. Jaiswal AK, et al. 2020. Immunobiology. 225:151956. PubMed
  6. Baryawno N et al. 2019. Cell. 177(7):1915-1932 . PubMed
  7. Chen J, et al. 2021. Sci Adv. 7:. PubMed
  8. Pessoa Rodrigues C, et al. 2021. Sci Adv. 7:. PubMed
  9. Al-Rifai R, et al. 2022. Nat Commun. 13:6592. PubMed
  10. Forte D, et al. 2020. Cell Metab. 32:829. PubMed
  11. Sykes DB et al. 2016. Cell. 167(1):171-186 . PubMed
  12. Mitchell K, et al. 2018. J Exp Med. 215:1709. PubMed
  13. Goldsmith JR, et al. 2020. Nat Commun. 11:2591. PubMed
  14. Fukushima T, et al. 2019. Cell Rep. 29:4144. PubMed
  15. Jia W, et al. 2021. Nat Commun. 12:2118. PubMed
  16. Park D, et al. 2020. Cancer Res. 80:4172. PubMed
  17. Kotfis K, et al. 2020. British Journal of Pharmacology. 49(1):66-72. PubMed
  18. La Rose AM, et al. 2021. Mol Metab. 53:101265. PubMed
  19. Mansell E, et al. 2020. Cell Stem Cell. . PubMed
  20. Elahi S, et al. 2020. Stem Cell Res. 43:101710. PubMed
  21. Yuzugullu H, et al. 2015. Nat Commun. 6: 8501. PubMed
  22. Runyan CE, et al. 2020. Aging Cell. 19:00. PubMed
  23. LaFleur MW, et al. 2019. Nat Commun. 10:1668. PubMed
  24. Zhou T, et al. 2022. Sci Adv. 8:eabj9617. PubMed
  25. Thongthip S, et al. 2016. Genes Dev. 30: 645 - 659. PubMed
  26. Nijnik A, et al. 2012. Blood. 119:1522. PubMed
  27. Koide S, et al. 2022. iScience. 25:103603. PubMed
  28. Chavez JS, et al. 2022. Cells. 11:. PubMed
  29. Yamashita M, et al. 2019. Cell Stem Cell. 25:357. PubMed
  30. Evavold CL, et al. 2021. Cell. 184(17):4495-4511.e19. PubMed
  31. Radulovic V, et al. 2020. Cell Reports. 27(10):2826-2836.e5.. PubMed
  32. Severe N et al. 2019. Cell Stem Cell. 25(4):570-583 . PubMed
  33. Cui Y, et al. 2020. Eur J Pharmacol. 881:173233. PubMed
  34. Dingler FA, et al. 2020. Mol Cell. . PubMed
  35. Pessoa Rodrigues C, et al. 2020. Sci Adv. 6:eaaz4815. PubMed
  36. Reyna DE et al. 2017. Cancer cell. 32(4):490-505 . PubMed
RRID
AB_10898327 (BioLegend Cat. No. 108127) AB_2563064 (BioLegend Cat. No. 108128)

Antigen Details

Structure
Ly-6 multigene family, 18 kD
Distribution

Hematopoietic stem cells, activated T cells and B cells, subset of resting B cells and T cells

Function
Regulates B and T cell responses
Cell Type
B cells, Hematopoietic stem and progenitors, Mesenchymal Stem Cells, T cells
Biology Area
Immunology, Stem Cells
Antigen References

1. Rock KL, et al. 1989. Immunol. Rev. 111:195.
2. Morrison SJ, et al. 1994. Immunity 1:661.
3. Spangrude GJ, et al. 1988. J. Immunol. 141:3697.
4. Malek T, et al. 1986. J. Exp. Med. 164:709.

Gene ID
110454 View all products for this Gene ID
UniProt
View information about Ly-6A/E on UniProt.org

联系我们

TEL：021-34661275 　点击拨打热线