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首页 > 抗体蛋白 > 抗体
APC/Cyanine7 anti-mouse Ly-6A/E (Sca-1) Antibody
产品名称：
APC/Cyanine7 anti-mouse Ly-6A/E (Sca-1) Antibody
产品类别：
抗体
产品编号：
108125
产品应用：
108125
[价格]
规格 价格 库存
25µg ￥ 1414 1

产品详情

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
IL-2-dependent mouse T-cell line (CTL-L)
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with APC/Cyanine7 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

The D7 antibody has been reported to induce T cell activation and inhibit TCR-induced IL-2 production. Additional reported applications (for the relevant formats) include: Western blotting1,2, immunoprecipitation1, in vitro lymphocyte activation3-6, induction of redirected lysis7, induction of T cell inhibitory signalling8, immunofluorescence9, and immunohistochemical staining of acetone-fixed frozen sections13 and Bouin-fixed,?paraffin-embedded samples9.

The two Sca-1 recognizing clones?D7?and E13-161.7 have been shown to bind distinct epitopes due to the inability of D7 to block the binding of E13-161.7.14?

Additional Product Notes
BioLegend is in the process of converting the name APC/Cy7 to APC/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance from our APC/Cyanine7 products. Please contact Technical Service if you have any questions.
Application References

(PubMed link indicates BioLegend citation)
  1. Ortega G, et al. 1986. J. Immunol. 137:3240. (WB, IP)
  2. Palfree RGE, et al. 1986. Immunogenetics 23:197. (WB)
  3. Codias EK, et al. 1990. J. Immunol. 144:2197.
  4. Malek TR, et al. 1986. J. Exp. Med. 164:709.
  5. Codias EK, et al. 1990. J. Immunol. 145:1407.
  6. Ivanov V, et al. 1994. J. Immunol. 153:2394.
  7. Karlhofer FM, et al. 1991. J. Immunol. 146:3662.
  8. Fleming T, et al. 1994. J. Immunol. 153:1955.
  9. van Bragt MPA, et al. 2005. Biol. Reprod. 73:634. (IF, IHC)
  10. Umland O, et al. 2007. J. Immunol. 178:4147.
  11. Cridland SO, et al. 2009. Blood Cell. Mol. Dis. 45:149. (FC) PubMed
  12. Pronk CJ, et al. 2011. J. Exp Med. PubMed
  13. English A, et al. 2000. J. Immunol. 165:3763. (IHC)
  14. Bamezai A and Rock KL. 1995. Proc. Natl. Acad. Sci. USA 92:4294.
  15. Wiesner DL, et al. 2015. PLoS Pathog. 11:1004701. PubMed
Product Citations
  1. Lawson H, et al. 2021. Stem Cell Reports. 16:2784. PubMed
  2. Barros-Silva JD, et al. 2018. Cell Rep. 25:3504. PubMed
  3. Abraham A, et al. 2019. J Clin Invest. 130:2685. PubMed
  4. Sch?nberger K, et al. 2022. Cell Stem Cell. 29:131. PubMed
  5. Sharma GP, et al. 2021. PLoS One. 16:e0259042. PubMed
  6. Corna G, et al. 2016. J Immunol. 197: 1914 - 1925. PubMed
  7. Paris J et al. 2019. Cell Stem Cell. 25(1):137-148 . PubMed
  8. Chen X, et al. 2021. Theranostics. 11:4655. PubMed
  9. Larsen SB, et al. 2021. Cell Stem Cell. :. PubMed
  10. Kobayashi T, et al. 2019. Cell. 176:982. PubMed
  11. Nawaz A, et al. 2022. Nat Commun. 13:7058. PubMed
  12. Fu X, et al. 2015. J Biol Chem. 290: 26445 - 26456. PubMed
  13. Zong L, et al. 2021. NPJ Aging Mech Dis. 7:25. PubMed
  14. Valds-Mora F, et al. 2021. Cell Reports. 35(2):108945. PubMed
  15. Nakamura‐Ishizu A et al. 2018. Cell reports. 25(7):1772-1785 . PubMed
  16. Jakob L, et al. 2021. Int J Mol Sci. 22:. PubMed
  17. Joshi PA, et al. 2019. Nat Commun. 10:1760. PubMed
  18. Hillel–Karniel C, et al. 2020. Cell Reports. 30(3):807-819.e4.. PubMed
  19. McAlpine CS, et al. 2021. Nature. 595:701. PubMed
  20. Grandl G, et al. 2016. Mol Metab. 5:937-947. PubMed
  21. JI B, et al. 2016. Cell Death Differ. 23:759-75. PubMed
  22. Yang SH, et al. 2017. Front Immunol. 8:1192. PubMed
  23. Gomzikova MO, et al. 2020. Sci Rep. 10:10740. PubMed
  24. Carr M, et al. 2016. Proc Natl Acad Sci U S A. 113(52):15024-15029. PubMed
  25. Reismann D, et al. 2017. Nat Commun.. 10.1038/s41467-017-01538-9. PubMed
  26. Helbling PM, et al. 2019. Cell Rep. 29:3313. PubMed
  27. Hermetet F, et al. 2019. Nat Commun. 10:523. PubMed
  28. Koyama T, et al. 2022. Curr Issues Mol Biol. 44:3146. PubMed
  29. Ye H, et al. 2021. Cancer Discov. 0.805555556. PubMed
  30. Agarwal P, et al. 2019. Cell Stem Cell. 24:769. PubMed
  31. Matsumura T, et al. 2022. Nat Commun. 13:7064. PubMed
  32. Sakamoto K, et al. 2021. Immunity. 54:2321. PubMed
  33. Cai Z, et al. 2020. Cell Rep. 31:107816. PubMed
  34. Vasamsetti SB, et al. 2018. Immunity. 49:93. PubMed
  35. Sch?nberger K, et al. 2022. STAR Protoc. 3:101408. PubMed
  36. Bellomo A, et al. 2020. Immunity. 53(1):127-142.e7. PubMed
  37. Ozaki M, et al. 2021. J Stem Cells Regen Med. 16:50. PubMed
  38. Rai S, et al. 2022. Nat Commun. 13:5346. PubMed
  39. Jie Z, et al. 2014. J Immunol. 192:3289. PubMed
  40. Sakamoto K, et al. 2022. STAR Protoc. 3:101052. PubMed
  41. Jatho A, et al. 2022. Cells. 11:. PubMed
  42. Fast EM, et al. 2021. Elife. 10:. PubMed
  43. Hu B, et al. 2020. J Clin Invest. 130:3483. PubMed
  44. Heyde A, et al. 2021. Cell. 184(5):1348-1361.e22. PubMed
  45. Agarwal P, et al. 2021. Cell Reports. 36(2):109386. PubMed
  46. Gross KM, et al. 2019. Cell Rep. 28:394. PubMed
RRID
AB_10639725 (BioLegend Cat. No. 108125) AB_10645327 (BioLegend Cat. No. 108126)

Antigen Details

Structure
Ly-6 multigene family, 18 kD
Distribution

Hematopoietic stem cells, activated T cells and B cells, subset of resting B cells and T cells

Function
Regulates B and T cell responses
Cell Type
B cells, Hematopoietic stem and progenitors, Mesenchymal Stem Cells, T cells
Biology Area
Immunology, Stem Cells
Antigen References

1. Rock KL, et al. 1989. Immunol. Rev. 111:195.
2. Morrison SJ, et al. 1994. Immunity 1:661.
3. Spangrude GJ, et al. 1988. J. Immunol. 141:3697.
4. Malek T, et al. 1986. J. Exp. Med. 164:709.

Gene ID
110454 View all products for this Gene ID
UniProt
View information about Ly-6A/E on UniProt.org

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