Pacific Blue™ anti-mouse CD49b (pan-NK cells
- 产品名称：
- Pacific Blue™ anti-mouse CD49b (pan-NK cells
- 产品类别：
- 抗体
- 产品编号：
- 108917
- 产品应用：
- 108917
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- IL-2-propagated NK1.1+ cells from C57BL/6 mice
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography, and conjugated with Pacific Blue? under optimal conditions.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 ?g per million cells in 100 ?l volume or 100 ?l of whole blood.? It is recommended that the reagent be titrated for optimal performance for each application.
* Pacific Blue? has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue? conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.
Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Excitation Laser
- Violet Laser (405 nm)
- Application Notes
The DX5 clone detects cells expressing relatively high levels of CD49b and may not be useful for the detection of cells expressing low levels of CD49b. DX5 does not block NK cell killing or binding to collagen in vitro. Additional reported applications (for the relevant formats) include: complement-mediated cytotoxicity2 and immunohistochemical staining5 of formalin-fixed and paraffin-embedded tissue sections as well as immunohistochemical staining of acetone-fixed frozen sections10. The binding of DX5 antibody to splenic NK cells can be blocked by HMa2 antibody.
- Application References
(PubMed link indicates BioLegend citation) -
- Arase H, et al. 2001. J. Immunol. 167:1141. (FC)
- Sepulveda H, et al. 1999. J. Immunol. 163:1133.
- Norian LA and Allen PM. 2004. J. Immunol. 173:835. (FC)
- Andoniou CE, et al. 2005. Nature Immunology 6:1011.
- Oertelt S, et al. 2006. J. Immunol. 177:1655. (IHC) PubMed
- Bourdeau A, et al. 2007. Blood doi:10.1182/blood-2006-08-044370.
- Charles N, et al. 2010. Nat. Med. 16:701. (FC) PubMed
- Qui Q, et al. 2010. J. Immunol. 184:1681. (FC) PubMed
- Busche A, et al. 2011. J. Immunol. 186:2918. PubMed
- Kim HR, et al. 2011. Nephrology 16:545. (IHC) PubMed
- Seyoum B, et al. 2011. Vaccine. 29:8002. PubMed
- Younos IH, et al. 2012. Int Immunopharmacol. 13:245. PubMed
- Honjo K, et al. 2012. PNAS. PubMed.
- Huang HN, et al. 2013. Biomaterials. 34:10151. PubMed
- Product Citations
-
- Quinn K, et al. 2013. J Immunol. 191:5085. PubMed
- Schneider C, et al. 2018. Cell. 174:271. PubMed
- Ciecko AE, et al. 2019. Cell Rep. 29:3073. PubMed
- Deady L, et al. 2014. Infect Immun . 82:1982. PubMed
- Flommersfeld S, et al. 2021. Immunity. :. PubMed
- Saravia J, et al. 2015. PLoS Pathog. 11: e1005217. PubMed
- Wiedemann GM, et al. 2020. Cell Rep. 33:108498. PubMed
- Lewis EL, et al. 2021. Front Immunol. 12:741518. PubMed
- Brown CC, et al. 2020. Cell. 179(4):846-863.e24.. PubMed
- Cohen M et al. 2018. Cell. 175(4):1031-1044 . PubMed
- Yeung F, et al. 2020. Cell Host & Microbe. 27(5):809-822. PubMed
- Dahlgren MW et al. 2019. Immunity. 50(3):707-722 . PubMed
- Yu X, et al. 2016. Blood. 127: 132 - 138. PubMed
- Sheppard S, et al. 2021. Cell Reports. 35(9):109210. PubMed
- Geary CD et al. 2018. Cell reports. 24(8):1949-1957 . PubMed
- RRID
- AB_2249376 (BioLegend Cat. No. 108917) AB_2265144 (BioLegend Cat. No. 108918)
- Structure
- Integrin α chain, 150 kD
- Distribution
-
NK cells, subset of T cells
- Function
- Adhesion
- Ligand/Receptor
- Collagen, laminin
- Cell Type
- NK cells, T cells
- Biology Area
- Cell Adhesion, Cell Biology, Immunology, Innate Immunity
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
-
1. Arase H, et al. 2001. J. Immunol. 167:1141.
2. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
3. Sasaki K, et al. 2003. Int. Immunol. 15:701.
4. Inoue O, et al. 2003. J. Cell Biol. 160:769.
- Gene ID
- 16398 View all products for this Gene ID
- UniProt
- View information about CD49b on UniProt.org
