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APC anti-mouse Ly-6A/E (Sca-1) Antibody
产品名称：
APC anti-mouse Ly-6A/E (Sca-1) Antibody
产品类别：
抗体
产品编号：
108111
产品应用：
108111
[价格]
规格 价格 库存
25µg ￥ 1162 1

产品详情

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
IL-2-dependent mouse T-cell line (CTL-L)
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

The D7 antibody has been reported to induce T cell activation and inhibit TCR-induced IL-2 production. Additional reported applications (for the relevant formats) include: Western blotting1,2, immunoprecipitation1, in vitro lymphocyte activation3-6, induction of redirected lysis7, induction of T cell inhibitory signalling8, immunofluorescence9, and immunohistochemical staining of acetone-fixed frozen sections13 and Bouin-fixed,?paraffin-embedded samples9.

The two Sca-1 recognizing clones?D7?and E13-161.7 have been shown to bind distinct epitopes due to the inability of D7 to block the binding of E13-161.7.14?

Application References

(PubMed link indicates BioLegend citation)
  1. Ortega G, et al. 1986. J. Immunol. 137:3240. (WB, IP)
  2. Palfree RGE, et al. 1986. Immunogenetics 23:197. (WB)
  3. Codias EK, et al. 1990. J. Immunol. 144:2197.
  4. Malek TR, et al. 1986. J. Exp. Med. 164:709.
  5. Codias EK, et al. 1990. J. Immunol. 145:1407.
  6. Ivanov V, et al. 1994. J. Immunol. 153:2394.
  7. Karlhofer FM, et al. 1991. J. Immunol. 146:3662.
  8. Fleming T, et al. 1994. J. Immunol. 153:1955.
  9. van Bragt MPA, et al. 2005. Biol. Reprod. 73:634. (IF, IHC)
  10. Umland O, et al. 2007. J. Immunol. 178:4147.
  11. Cridland SO, et al. 2009. Blood Cell. Mol. Dis. 45:149. (FC) PubMed
  12. Pronk CJ, et al. 2011. J. Exp Med. PubMed
  13. English A, et al. 2000. J. Immunol. 165:3763. (IHC)
  14. Bamezai A and Rock KL. 1995. Proc. Natl. Acad. Sci. USA 92:4294.
  15. Wiesner DL, et al. 2015. PLoS Pathog. 11:1004701. PubMed
Product Citations
  1. Philpott J, et al. 2022. Immunohorizons. 6:366. PubMed
  2. Mikami Y, et al. 2021. Immunity. 54(3):514-525.e6. PubMed
  3. Umland O, et al. 2007. J Immunol . 178:4147. PubMed
  4. Ju W, et al. 2020. J Biomed Sci. 27:91. PubMed
  5. Viny AD, et al. 2019. Cell Stem Cell. 25:682. PubMed
  6. Shi Y, et al. 2020. Med Sci Monit. 26:e923328. PubMed
  7. Bo Yu et al. 2018. Cell stem cell. 23(2):193-209 . PubMed
  8. Nguyen N, et al. 2022. iScience. 25:103679. PubMed
  9. Hinterbrandner M, et al. 2021. JCI Insight. 6:e151797. PubMed
  10. Larouche JA, et al. 2021. eLife. 0.416666666666667. PubMed
  11. Fujino T, et al. 2021. Nat Commun. 12:1826. PubMed
  12. Liu X, et al. 2021. eLife. 0.416666666666667. PubMed
  13. Yamamoto K, et al. 2021. Cell Reports. 36(8):109576. PubMed
  14. Mann M, et al. 2018. Cell Rep. 25:2992. PubMed
  15. Yin N et al. 2019. Cancer Cell. 36(2):156-167 . PubMed
  16. Fukushima T, et al. 2019. Cell Rep. 29:4144. PubMed
  17. Endo Y, et al. 2020. FASEB J. 34:16086. PubMed
  18. Vannini N, et al. 2019. Cell Stem Cell. 24:405. PubMed
  19. De S, et al. 2018. Development. 145. PubMed
  20. Wiesner D, et al. 2015. PLoS Pathog. 11:1004701. PubMed
  21. Kunimoto H, et al. 2018. Cancer Cell. 33:44. PubMed
  22. Hillel–Karniel C, et al. 2020. Cell Reports. 30(3):807-819.e4.. PubMed
  23. Shcherbina A, et al. 2020. Cell Rep. 32:107964. PubMed
  24. Morimoto A, et al. 2021. Nat Commun. 12:2136. PubMed
  25. Ferreirinha P, et al. 2022. Development. 149:. PubMed
  26. Derecka M, et al. 2020. Nat Immunol. 261:21. PubMed
  27. Jungwirth N et al. 2018. Journal of molecular neuroscience : MN. 65(1):60-73 . PubMed
  28. Hou M, et al. 2020. Stem Cell Res Ther. 11:55. PubMed
  29. Tavakol DN, et al. 2021. Curr Protoc. 1:e275. PubMed
  30. Hepler C et al. 2018. eLife. 7 pii: e39636. PubMed
  31. Ikram M, et al. 2018. Oncotarget. 9:2058. PubMed
  32. Mizbani A, et al. 2016. Development. 143: 4137 - 4148. PubMed
  33. Ding P, et al. 2022. Bone Res. 10:42. PubMed
  34. Gentek R, et al. 2018. Immunity. 48:1160. PubMed
  35. Goldstein JM et al. 2019. Cell reports. 27(4):1254-1264 . PubMed
  36. Luo H, et al. 2020. Cancer Cell. 36(6):645-659.e8.. PubMed
  37. Luo H, et al. 2022. Mol Cell. 82:833. PubMed
  38. Keerthivasan S, et al. 2021. Immunity. 54(7):1511-1526.e8. PubMed
  39. Bastianutto C, et al. 2007. Cancer Res. 67:10112. PubMed
  40. Glotzbach J, et al. 2011. PLoS One. 6:e21211. PubMed
  41. Wolock SL, et al. 2019. Cell Rep. 28:302. PubMed
  42. Rodríguez L, et al. 2021. Biomolecules. 11: . PubMed
  43. Singhal P, et al. 2016. Proc Natl Acad Sci U S A. 113: 122 - 127. PubMed
  44. Markworth JF, et al. 2020. JCI Insight. 5:00. PubMed
  45. Wong J, et al. 2016. Sci Signal. 9: ra103. PubMed
  46. Vannini N, et al. 2016. Nat Commun. 7:13125. PubMed
  47. Vogiatzi A, et al. 2021. Mol Cell Biol. 41:e0014921. PubMed
  48. Liu Q, et al. 2016. Cell Death Dis. 1.93125. PubMed
  49. Zhang Y, et al. 2008. Mol Cell Biol. 28:1688. PubMed
  50. Thomson CA, et al. 2018. J Immunol. 201:215. PubMed
  51. Cohen I et al. 2018. Cell stem cell. 22(5):726-739 . PubMed
RRID
AB_313348 (BioLegend Cat. No. 108111) AB_313349 (BioLegend Cat. No. 108112)

Antigen Details

Structure
Ly-6 multigene family, 18 kD
Distribution

Hematopoietic stem cells, activated T cells and B cells, subset of resting B cells and T cells

Function
Regulates B and T cell responses
Cell Type
B cells, Hematopoietic stem and progenitors, Mesenchymal Stem Cells, T cells
Biology Area
Immunology, Stem Cells
Antigen References

1. Rock KL, et al. 1989. Immunol. Rev. 111:195.
2. Morrison SJ, et al. 1994. Immunity 1:661.
3. Spangrude GJ, et al. 1988. J. Immunol. 141:3697.
4. Malek T, et al. 1986. J. Exp. Med. 164:709.

Gene ID
110454 View all products for this Gene ID
UniProt
View information about Ly-6A/E on UniProt.org

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