Product Details

Product Information

Intended Use:

In Vitro Diagnostic (IVD). Use in immunohistochemistry (IHC) test methods only.

The monoclonal antibody D2-40 is used for the in vitro examination of frozen or paraffin-embedded tissue sections of human bowel or esophagus?samples using immunohistochemistry (IHC) methods for the qualitative identification of D2-40 Lymphatic Endothelial Marker. The clinical interpretation of any staining or its absence should be complemented by morphological studies and proper controls and should be evaluated within the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.

Reactivity

Human

Immunogen

This antibody was developed using M2A.

Formulation

Phosphate-buffered solution with BSA + 0.1% NaN₃.

Preparation

Ammonium sulfate precipitated TCS.

Storage & Handling

Store between 2°C and 8°C.

Recommended Usage

Each lot of this antibody is quality control tested by immunohistochemical staining of formalin-fixed paraffin-embedded sections of human bowel and esophagus. Frozen human sections of bowel and esophagus have been verified during product development.

The optimal working dilution should be determined for each specific assay condition.
? IHC: ≥1:40 (concentrated format) for Biotin based detection systems such as USA Ultra Streptavidin Detection (Cat. No. 929501).

Tissue Sections: Formalin-fixed, paraffin-embedded tissues, frozen sections
Pretreatment: Not required
Incubation: 60 minutes at room temperature

Application References

(PubMed link indicates BioLegend citation)

1. Hamanaka T, et al. 2011. Invest. Ophthalmol. Vis. Sci. 52:8849. (IHC) PubMed
2. Choi WW, et al. 2005. Mod Pathol. 18:143. PubMed
3. Chu AY, et al. 2005. Mod Pathol. 18:105.
4. Dumoff KL, et al. 2005. Mod Pathol. 18:97.
5. Galambos C, Nodit L. 2005. Ped Dev Pathol. 8:181.
6. Fogt F, et al. 2004. Oncol Rep. 11:47.
7. Fogt F, et al. 2004. Int J Molec Med. 13:681.

RRID

AB_2565185 (BioLegend Cat. No. 916604) AB_2565182 (BioLegend Cat. No. 916601) AB_2565184 (BioLegend Cat. No. 916603) AB_2565183 (BioLegend Cat. No. 916602)

Disclaimer

WARNINGS AND PRECAUTIONS

1. Use appropriate personal protective equipment and safety practices per universal precautions when working with this reagent. Refer to the reagent safety data sheet.
2. This antibody contains sodium azide. Follow federal, state and local regulations to dispose of this reagent. Sodium azide build-up in metal wastepipes may lead to explosive conditions; if disposing of reagent down wastepipes, flush with water after disposal.
3. All specimens, samples and any material coming in contact with them should be considered potentially infectious and should be disposed of with proper precautions and in accordance with federal, state and local regulations.
4. Do not use this reagent beyond the expiration date stated on the label.
5. Do not use this reagent if it appears cloudy or if there is any change in the appearance of the reagent as these may be indication of possible deterioration.