PE/Cyanine7 anti-human CD158b/j (KIR2DL2/L3/S2) An-上海雅吉生物科技有限公司

规格	价格	库存
25tests	￥ 2058	1

Product Details

Verified Reactivity

Human

Antibody Type

Monoclonal

Host Species

Mouse

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)

Preparation

The antibody was purified by affinity chromatography and conjugated with PE/Cyanine7 under optimal conditions.

Concentration

Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood.

Excitation Laser

Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)

Application Notes

The DX27 monoclonal antibody reacts with a common epitope of KIR2DL2 (CD158b1, p58.2), KIR2DL3 (CD158b2, p58.3), and KIR2DS2 (CD158j, p50.2). Additional reported applications (for the relevant formats) include: restoring the NK cell cytotoxicity^1,5.

This clone has been tested in-house and determined to not be suitable for applications in immunohistochemistry of paraffin-embedded tissue sections (IHC-P).

Additional Product Notes

BioLegend is in the process of converting the name PE/Cy7 to PE/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance from our PE/Cyanine7 products. Please contact Technical Service if you have any questions.

Application References (PubMed link indicates BioLegend citation)

Bakker ABH, et al. 1998. J. Immunol. 160:5239.
Lucas M, et al. 2003. J. Virol. 77:2251.
Goodier M, et al. 2000. J. Immunol. 165:139.
Yawata M, et al. 2002. Immunogenetics 54:543.
Valiante NM, et al. 1997. Immunity 7:739.

Product Citations

Palamides P, et al. 2016. Dis Model Mech. 9: 985 - 997. PubMed
Nguyen MAT, et al. 2020. Immunohorizons. 0.465277778. PubMed
Dulberger CL et al. 2017. Immunity. 46(6):1018-1029 . PubMed

RRID

AB_2563374 (BioLegend Cat. No. 312609) AB_2563375 (BioLegend Cat. No. 312610)

Antigen Details

Structure: Immunoglobulin superfamily member, contains two immunoglobulin C2-type domains, type I membrane protein
Distribution: NK cells and a subset of T cells
Function: Inhibits cytotoxic function of NK and T cells upon interacting with specific HLA-C antigens on target cell
Ligand/Receptor: HLA-C antigens such as HLA-Cw1, HLA-Cw3, HLA-Cw7
Cell Type: NK cells, T cells
Biology Area: Immunology
Molecular Family: CD Molecules
Antigen References: 1. Colonna M, et al. 1995. Science 268:405.
2. Uhrburg M, et al. 1997. Immunity 7:753.
3. Wagtmann N, et al. 1995. Immunity 3:801.
4. Dohring C, et al. 1996. Immunogenetics 44:227.
5. Maenaka K, et al. 1999. Structure 7:391.
Gene ID: 3803 View all products for this Gene ID
UniProt: View information about CD158b/j on UniProt.org

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