Biotin anti-human C3a/C3a(desArg)/C3 Antibody
- 产品名称:
- Biotin anti-human C3a/C3a(desArg)/C3 Antibody
- 产品类别:
- 抗体
- 产品编号:
- 518001
- 产品应用:
- 518001
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Human C3a
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
- Application
-
ELISA Detection - Quality tested
WB - Reported in the literature, not verified in house
- Recommended Usage
Each lot of this antibody is quality control tested by ELISA assay. For ELISA detection applications, a concentration range of 0.5-2 ?g/ml is recommended. To obtain a linear standard curve, serial dilutions of C3a recombinant protein ranging from?39 to 2500 pg/ml are recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
The D17/1 antibody reacts with human C3a, C3a(desArg) and C3. The D17/1 antibody can neutralize the bioactivity of natural or recombinant C3a.
ELISA Detection: The biotinylated D17/1 antibody is useful as the detection antibody in a sandwich ELISA assay, when used in conjunction with the purified K13/16 antibody (Cat. No. 518102) as the capture antibody.
- Application References
(PubMed link indicates BioLegend citation) -
- Oppermann M, et al. 1991. Kidney Int. 40:939947.
- Oppermann M, et al. 1992. Immunopharm. 24:119134.
- Elsner J, et al. 1994. Blood 83:3324.
- RRID
- AB_10896912 (BioLegend Cat. No. 518001) AB_10895750 (BioLegend Cat. No. 518002)
- Ligand/Receptor
- C3a bind to C3a receptor, C3a desArg bind to C5L2 (GPR77)
- Biology Area
- Cell Biology, Complement, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
- Antigen References
-
1. de Bruijn MH, et al. 1985. P. Natl. Acad. Sci. USA 82:708.
2. Hugli TE, et al. 1975. J. Biol. Chem. 250:8293.
3. Oxvig C, et al. 1995. J. Biol. Chem. 270:13645.
- Gene ID
- 718 View all products for this Gene ID
- UniProt
- View information about C3a/C3adesArg/C3 on UniProt.org