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Biotin anti-human C3a/C3a(desArg)/C3 Antibody
产品名称:
Biotin anti-human C3a/C3a(desArg)/C3 Antibody
产品类别:
抗体
产品编号:
518001
产品应用:
518001
[价格]
规格 价格 库存
50µg ¥ 1568 1

产品详情

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human C3a
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application

ELISA Detection - Quality tested
WB - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by ELISA assay. For ELISA detection applications, a concentration range of 0.5-2 ?g/ml is recommended. To obtain a linear standard curve, serial dilutions of C3a recombinant protein ranging from?39 to 2500 pg/ml are recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

The D17/1 antibody reacts with human C3a, C3a(desArg) and C3. The D17/1 antibody can neutralize the bioactivity of natural or recombinant C3a.

ELISA Detection: The biotinylated D17/1 antibody is useful as the detection antibody in a sandwich ELISA assay, when used in conjunction with the purified K13/16 antibody (Cat. No. 518102) as the capture antibody.

Application References

(PubMed link indicates BioLegend citation)
  1. Oppermann M, et al. 1991. Kidney Int. 40:939947.
  2. Oppermann M, et al. 1992. Immunopharm. 24:119134.
  3. Elsner J, et al. 1994. Blood 83:3324.
RRID
AB_10896912 (BioLegend Cat. No. 518001) AB_10895750 (BioLegend Cat. No. 518002)

Antigen Details

Ligand/Receptor
C3a bind to C3a receptor, C3a desArg bind to C5L2 (GPR77)
Biology Area
Cell Biology, Complement, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
Antigen References

1. de Bruijn MH, et al. 1985. P. Natl. Acad. Sci. USA 82:708.
2. Hugli TE, et al. 1975. J. Biol. Chem. 250:8293.
3. Oxvig C, et al. 1995. J. Biol. Chem. 270:13645.

Gene ID
718 View all products for this Gene ID
UniProt
View information about C3a/C3adesArg/C3 on UniProt.org

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