首页 > 抗体蛋白 > 抗体
PE anti-human CD94 Antibody
产品名称:
PE anti-human CD94 Antibody
产品类别:
抗体
产品编号:
305506
产品应用:
305506
[价格]
规格 价格 库存
100tests ¥ 3416 1

产品详情

Product Details

Verified Reactivity
Human
Reported Reactivity
African Green, Baboon, Cynomolgus
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
NK cell line
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
SB - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation4, inhibition of NK cell-mediated lysis5,?immunohistochemical staining of acetone-fixed frozen tissue sections, and spatial biology (IBEX)6,7.

Additional Product Notes

Iterative Bleaching Extended multi-pleXity (IBEX) is a fluorescent imaging technique capable of highly-multiplexed spatial analysis. The method relies on cyclical bleaching of panels of fluorescent antibodies in order to image and analyze many markers over multiple cycles of staining, imaging, and, bleaching. It is a community-developed open-access method developed by the Center for Advanced Tissue Imaging (CAT-I) in the National Institute of Allergy and Infectious Diseases (NIAID, NIH).

Application References

(PubMed link indicates BioLegend citation)
  1. Mizuki M, et al. 2000. Sarcoidosis Vasc. Diffuse Lung Dis. 17:54.
  2. Phillip J, et al. 1996. Immunity 5:163.
  3. Lazetic S, et al. 1996. J. Immunol. 157:4741.
  4. Lanier LL, et al. 1998. Immunity 8:693.
  5. Wooden SL, et al. 2005. J. Immunol. 175:1383.
  6. Radtke AJ,?et al. 2020.?Proc Natl Acad Sci USA. 117:33455-33465. (SB)?PubMed
  7. Radtke AJ,?et al. 2022.?Nat Protoc. 17:378-401. (SB)?PubMed
Product Citations
  1. Péguillet I, et al. 2014. Cancer Res. 74:2204. PubMed
  2. Chen M, et al. 2021. Cancers (Basel). 13:. PubMed
  3. Palamides P, et al. 2016. Dis Model Mech. 9: 985 - 997. PubMed
  4. Mold JE, et al. 2021. Cell Reports. 35(8):109174. PubMed
RRID
AB_314536 (BioLegend Cat. No. 305506)

Antigen Details

Structure
C-type lectin, type II transmembrane glycoprotein, covalently associates with NKG2, 43 kD
Distribution

NK cells , T subset

Function
Inhibits NK function
Cell Type
NK cells, T cells
Biology Area
Immunology, Innate Immunity
Molecular Family
CD Molecules
Antigen References

1. Lopez-Botet M, et al. 1997. Immunol. Rev. 155:165.
2. Moretta A, et al. 1997. Immunol. Rev. 155:105.

Gene ID
3824 View all products for this Gene ID
UniProt
View information about CD94 on UniProt.org

联系我们

TEL:021-34661275  点击拨打热线