Ultra-LEAF™ Purified anti-human CD49d Antibo-上海雅吉生物科技有限公司

规格	价格	库存
1mg	￥ 9394	1

Product Details

Verified Reactivity

Human, Cynomolgus, Rhesus

Reported Reactivity

African Green, Baboon, Cat, Cow, Chimpanzee, Common Marmoset, Dog, Horse, Sheep, Squirrel Monkey

Antibody Type

Monoclonal

Host Species

Mouse

Formulation

0.2 ?m filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is <0.01 EU/?g of the protein (<0.001 ng/?g of the protein) as determined by the LAL test.

Preparation

The Ultra-LEAF? (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.

Concentration

The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF? solution contains no preservative; handle under aseptic conditions.

Application

FC - Quality tested
IHC-F, Costim - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.125 ?g per million cells in 100 ?l volume or 100 ?l of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections, and in vitro T cell costimulation^2,3. The Ultra-LEAF? Purified antibody (Endotoxin < 0.01 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays?(Cat. No. 304339 and 304340).

Application References (PubMed link indicates BioLegend citation)

Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
Jeong SH, et al. 2004. J. Virol. 78:6995. (Costim)
Vogel TU, et al. 2002. J. Immunol. 169:4511. (Costim)
Kleinewietfeld M, et al. 2009. Blood 113:827. (FC) PubMed
Palacious F, et al. 2010. Blood 115:4488. PubMed
Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
Sestak K, et al. 2007. Vet. Immunol. Immunopathol. 119:21.
Mattapallil MJ, et al. 2011. J. Immunol. 187:1977. PubMed

Product Citations

Pozzetto B, et al. 2021. Nature. 600:701. PubMed
Naaber P, et al. 2022. Cell Rep Med. 3:100716. PubMed
Fuseini H, et al. 2022. Front Immunol. 12:796898. PubMed
Olafsdottir TA, et al. 2022. Commun Biol. 5:914. PubMed

RRID

AB_2810443 (BioLegend Cat. No. 304339) AB_2810444 (BioLegend Cat. No. 304340)

Antigen Details

Structure: Integrin, type I transmembrane glycoprotein, 150 kD.
Distribution: T cells, B cells, NK , dendritic cells, thymocytes, monocytes, eosinophils, mast cells.
Function: Lymphocyte migration, T cell activation, stem cell differentiation.
Ligand/Receptor: Fibronectin, VCAM-1, MAdCAM-1
Cell Type: B cells, Dendritic cells, Eosinophils, Mast cells, Monocytes, NK cells, T cells, Thymocytes, Tregs
Biology Area: Cell Adhesion, Cell Biology, Immunology, Innate Immunity
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Elices M, Ed.1995. Springer Semin. Immunopathol. 16(4).
2. Lobb RR and Helmer ME. et al. 1994. J. Clin. Invest. 94:1722.
Gene ID: 3676 View all products for this Gene ID
UniProt: View information about CD49d on UniProt.org

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