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首页 > 抗体蛋白 > 抗体
PerCP/Cyanine5.5 anti-mouse CD19 Antibody
产品名称：
PerCP/Cyanine5.5 anti-mouse CD19 Antibody
产品类别：
抗体
产品编号：
115533
产品应用：
115533
[价格]
规格 价格 库存
25µg ￥ 1246 1

产品详情

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse CD19-expressing K562 human erythroleukemia cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is =0.25 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunofluorescence7.

Additional Product Notes
BioLegend is in the process of converting the name PerCP/Cy5.5 to PerCP/Cyanine5.5. The dye molecule remains the same, so you should expect the same quality and performance from our PerCP/Cyanine5.5 products. Contact Technical Service if you have any questions.
Application References

(PubMed link indicates BioLegend citation)
  1. Shoham T, et al. 2003. J. Immunol. 171:4062. (FC)
  2. Goodyear CS, et al. 2004. J. Immunol. 172:2870. (FC)
  3. Kamimura D, et al. 2006. J. Immunol. 177:306. (FC)
  4. Andoniou CE, et al. 2005. Nat. Immunol. 6:1011. (FC)
  5. Lawson BR, et al. 2007. J. Immunol. 178:5366. (FC)
  6. Phan TG, et al. 2007. Nat. Immunol. 8:992. (FC)
  7. Hayashida K, et al. 2008. J. Biol. Chem. 283:19895. (IF) PubMed
  8. Charles N, et al. 2010. Nat. Med. 16:701. (FC) PubMed
  9. Bankoti J, et al. 2010. Toxicol. Sci. 115:422. (FC) PubMed
  10. Stadnisky MD, et al. 2011. Blood. 117:5133. (FC) PubMed
  11. Perlot T, et al. 2012. J. Immunol. 188:1201. (FC) PubMed
  12. Olive V, et al. 2013. Elife. 2:822. PubMed
  13. Miyai T, et al. 2014. PNAS. 111:11780. PubMed
Product Citations
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  6. Frisbee AL, et al. 2019. Nat Commun. 10:2712. PubMed
  7. Wei H, et al. 2021. Malar J. 20:89. PubMed
  8. Guzmán M, et al. 2021. Mucosal Immunol. 14:1347. PubMed
  9. Webb LMC, et al. 2021. Aging Cell. 20:e13295. PubMed
  10. Lancaster JN, et al. 2019. Nat Commun. 10:2220. PubMed
  11. Kobayashi T, et al. 2019. Cell. 176:982. PubMed
  12. Smith D, et al. 2011. PLoS One. 7:e1002284. PubMed
  13. Bratti M, et al. 2022. Front Immunol. 13:1029759. PubMed
  14. Gopinathan G, et al. 2022. Mol Cancer Ther. 21:1030. PubMed
  15. Waide ML, et al. 2020. Cell Rep. 33:108503. PubMed
  16. Yousif AS, et al. 2020. Immunity. 54(2):235-246.e5. PubMed
  17. Zhu X, et al. 2017. Arch Oral Biol. 10.1016/j.archoralbio.2017.03.010. PubMed
  18. Eastman A, et al. 2016. J Immunol . 194: 5999-6010. PubMed
  19. Wang X, et al. 2016. PLoS Pathog. 12: 1005367. PubMed
  20. Woodruff MC et al. 2018. Cell reports. 25(2):321-327 . PubMed
  21. Jtte BB, et al. 2021. iScience. 24(8):102833. PubMed
  22. Santiago M, et al. 2010. J Immunol. 185:1114. PubMed
  23. Paiva RS, et al. 2021. Eur J Immunol. 51:1968. PubMed
  24. Yoshihara E, et al. 2020. Nature. 606:586. PubMed
  25. Fachi JL, et al. 2020. J Exp Med. 217:00:00. PubMed
  26. Sochalska M, et al. 2016. Oncogene. 10.1038/onc.2016.362. PubMed
  27. Cuff AO, et al. 2020. Frontiers in Immunology. 1.930555556. PubMed
  28. Palathingal Bava E, et al. 2022. JCI Insight. 7:. PubMed
  29. Krementsov DN, et al. 2018. Front Immunol. 9:1622. PubMed
  30. Daniel CJ, et al. 2022. Mol Cancer Res. 20:1151. PubMed
  31. Wagage S, et al. 2014. J Immunol. 192:1661. PubMed
  32. Uddin MJ, et al. 2022. Mucosal Immunol. 15:165. PubMed
  33. McFarland AP, et al. 2021. Immunity. 54(6):1320-1337.e4. PubMed
  34. Sangesland M, et al. 2020. Cell Reports. 32(8):108065. PubMed
  35. White C, et al. 2015. J Immunol. 194:697. PubMed
  36. Ariyoshi Y, et al. 2016. Gene Ther. 10.1038/gt.2016.7. PubMed
  37. Faust HJ, et al. 2020. J Clin Invest. 130:5493. PubMed
  38. Zhu H, et al. 2019. Nat Commun. 10:1084. PubMed
  39. LaFleur MW, et al. 2019. Nat Commun. 10:1668. PubMed
  40. Ding P, et al. 2022. Bone Res. 10:42. PubMed
  41. Denny JE, et al. 2019. Sci Rep. 2.786111111. PubMed
  42. Hajas A, et al. 2013. Hum Immunol. 19:93. PubMed
  43. DiToro D, et al. 2020. Immunity. 650:52. PubMed
  44. Kim DK, et al. 2022. Nat Commun. 13:6292. PubMed
  45. Sakamoto K, et al. 2021. Immunity. 54:2321. PubMed
  46. Glasenapp A, et al. 2021. Circ Res. 129:369. PubMed
  47. Holzki J, et al. 2015. J Virol. 89: 9886 - 9895. PubMed
  48. Isvoranu G, et al. 2019. Oncol Lett. 17:4197. PubMed
  49. Maniati E, et al. 2020. Cell Rep. 30:525. PubMed
  50. Ghezraoui H, et al. 2018. Nature. 560:122. PubMed
  51. Fedele C, et al. 2021. J Exp Med. 218: . PubMed
  52. Heil J, et al. 2021. Nat Commun. 12:6963. PubMed
  53. Li S, et al. 2013. Virology. 443:143. PubMed
  54. Velazquez VM, et al. 2017. Mol Ther Methods Clin Dev. 0.277083333. PubMed
  55. Royzman D, et al. 2019. Front Immunol. 10:633. PubMed
  56. Peng V, et al. 2022. Nat Immunol. 23:619. PubMed
  57. Sakamoto K, et al. 2022. STAR Protoc. 3:101052. PubMed
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RRID
AB_2259869 (BioLegend Cat. No. 115533) AB_2072925 (BioLegend Cat. No. 115534)

Antigen Details

Structure
Ig superfamily, associates with CD21 and CD81, 95 kD
Distribution

Pro-B cells to mature B cells (during development), follicular dendritic cells

Function
Modulates B cell activation and differentiation
Ligand/Receptor
CD21, CD81, Leu-13
Cell Type
B cells, Dendritic cells
Biology Area
Costimulatory Molecules, Immunology
Molecular Family
CD Molecules
Antigen References

1. Fearon DT. 1993. Curr. Opin. Immunol. 5:341.
2. Krop I, et al. 1996. Eur. J. Immunol. 26:238.
3. Krop I, et al. 1996. J. Immunol. 157:48.
4. Tedder TF, et al. 1994. Immunol. Today 15:437.

Gene ID
12478 View all products for this Gene ID
UniProt
View information about CD19 on UniProt.org

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