热线电话: 021-34661275
产品分类/PRODUCT CLASSIFICATION

上海细胞库

人源细胞系| 稳转细胞系| 基因敲除株| 基因点突变细胞株| 基因过表达细胞株| 重组细胞系| 猪的细胞系| 马细胞系| 兔的细胞系| 犬的细胞系| 山羊的细胞系| 鱼的细胞系| 猴的细胞系| 仓鼠的细胞系| 狗的细胞系| 牛的细胞| 大鼠细胞系| 小鼠细胞系| 其他细胞系|

原代细胞

原代细胞| 永生化细胞|

细胞培养

血清| 无血清冻存| 细胞培养试剂盒| 普通培养基| 完全培养基| 细胞污染预防| 细胞污染清除| 细胞培养辅助试剂|

细胞服务

STR鉴定服务| ATCC代购服务|

酶联试剂盒

人ELISA试剂盒| 大鼠ELISA试剂盒| 小鼠ELISA试剂盒| 兔ELISA试剂盒| 猪ELISA试剂盒| 鱼ELISA试剂盒| 牛ELISA试剂盒| 犬ELISA试剂盒| 鸭ELISA试剂盒| 豚鼠ELISA试剂盒| 鸡ELISA试剂盒| 绵羊ELISA试剂盒| 山羊ELISA试剂盒| 仓鼠ELISA试剂盒| 植物ELISA试剂盒| 药残留类试剂盒| 其他ELISA试剂盒|

生化试剂盒

生化试剂盒| 微量法检测盒| 分光法检测盒| 胶体金检测盒|

PCR试剂盒

荧光探针PCR试剂盒| 染料荧光PCR试剂盒| 核酸扩增/纯化| 普通PCR检测试剂盒| 质粒菌种感受态|

抗体蛋白

蛋白多肽| 抗体| 免疫组化| 免疫球蛋白|

试剂耗材

标准试液| 科研试剂| 缓 冲 液| 实验耗材| 高效液相色谱对照品|

首页 > 抗体蛋白 > 抗体
Brilliant Violet 605™ anti-mouse CD45 Antibo
产品名称：
Brilliant Violet 605™ anti-mouse CD45 Antibo
产品类别：
抗体
产品编号：
103140
产品应用：
103140
[价格]
规格 价格 库存
500µL ￥ 6090 1

产品详情

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse thymus or spleen
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 605? under optimal conditions.
Concentration
?g sizes: 0.2 mg/mL
?L sizes: lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining with ?g, the suggested use of this reagent is ≤0.5 ?g per million cells in 100 ?l volume. For flow cytometric staining with ?l, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 605? excites at 405 nm and emits at 603 nm. The bandpass filter 610/20 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 605? is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet?.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

Clone 30-F11 reacts with all isoforms and both CD45.1 and CD45.2 alloantigens of CD45.

Additional reported applications (for relevant formats) include: immunoprecipitation3, complement-dependent cytotoxicity1,5,?immunohistochemistry (acetone-fixed frozen sections, zinc-fixed paraffin-embedded sections and formalin-fixed paraffin-embedded sections)4,6, Western blotting7, and spatial biology (IBEX)10,11. The Ultra-LEAF? purified antibody (Endotoxin < 0.01 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. No. 103163 and 103164).

Application References

(PubMed link indicates BioLegend citation)
  1. Podd BS, et al. 2006. J. Immunol. 176:6532. (FC, CMCD) PubMed
  2. Haynes NM, et al. 2007. J. Immunol. 179:5099. (FC)
  3. Ledbetter JA, et al. 1979. Immunol. Rev. 47:63. (IP)
  4. Simon DI, et al. 2000. J. Clin. Invest. 105:293. (IHC)
  5. Seaman WE. 1983. J. Immunol. 130:1713. (CMCD)
  6. Cornet A, et al. 2001. P. Natl. Acad. Sci. USA 98:13306. (IHC)
  7. Tsuboi S and Fukuda M. 1998. J. Biol. Chem. 273:30680. (WB) PubMed
  8. Liu F, et al. 2012. Blood. 119:3295. PubMed
  9. Pelletier AN, et al. 2012. J. Immunol. 188:5561. PubMed
  10. Radtke?AJ,?et al.?2020.?Proc Natl Acad Sci U S A.?117:33455-65. (SB)?PubMed
  11. Radtke?AJ,?et al.?2022.?Nat Protoc.?17:378-401. (SB)?PubMed
Product Citations
  1. Liang J, et al. 2021. Cancer Manag Res. 13:6977. PubMed
  2. Luo J, et al. 2022. J Nanobiotechnology. 20:228. PubMed
  3. Zhu Y, et al. 2022. Nat Commun. 13:4282. PubMed
  4. Wu MJ, et al. 2022. Cancer Discov. 12:812. PubMed
  5. Wong P, et al. 2021. STAR Protoc. 2:100262. PubMed
  6. Dalmas E et al. 2017. Immunity. 47(5):928-942 . PubMed
  7. Flamar AL, et al. 2020. Immunity. 52(4):606-619.e6.. PubMed
  8. Dando SJ, et al. 2021. Invest Ophthalmol Vis Sci. 62:10. PubMed
  9. Delvecchio FR, et al. 2021. Cell Mol Gastroenterol Hepatol. 12:1543. PubMed
  10. Israelow B, et al. 2020. bioRxiv. . PubMed
  11. Platt DJ, et al. 2021. Cell Reports. 35(6):109113. PubMed
  12. Boyd DF, et al. 2020. Nature. 587:466. PubMed
  13. Turner JA, et al. 2020. Immunity. 53:1202. PubMed
  14. Khamissi FZ, et al. 2022. Sci Adv. 8:eabm5900. PubMed
  15. Zou C, et al. 2020. Cell Rep. 33:108447. PubMed
  16. Marinaccio C, et al. 2021. Cancer Discovery. 11(6):1398-1410. PubMed
  17. Lee J, et al. 2020. Gut Microbes. 1:. PubMed
  18. Barry KC, et al. 2018. Nat Med. 24:1178. PubMed
  19. Chen J, et al. 2022. J Nanobiotechnology. 20:283. PubMed
  20. Gao Y, et al. 2020. Nat Cell Biol. 1064:22. PubMed
  21. Shao Y, et al. 2019. Diabetes. 68:2131. PubMed
  22. Huai W, et al. 2019. J Exp Med. 216:772. PubMed
  23. Chen JC et al. 2018. Cell systems. 7(1):92-103 . PubMed
  24. Skibba ME, et al. 2021. Respir Res. 22:315. PubMed
  25. AR P, et al. 2016. Circ Res. 118: 400-409. PubMed
  26. Schiller M, et al. 2021. Immunity. 54(5):1022-1036.e8. PubMed
  27. Lu SX, et al. 2021. Cell. . PubMed
  28. García Nores GD, et al. 2018. Nat Commun. 9:1970. PubMed
  29. Grizotte–Lake M, et al. 2018. Immunity. 49:1103. PubMed
  30. Sharma S, et al. 2015. J Immunol. 194:5529. PubMed
  31. Zhang H, et al. 2020. Cancer Cell. 37(1):37-54.e9.. PubMed
  32. Nording H, et al. 2021. Nat Commun. 12:3352. PubMed
  33. Wang F, et al. 2021. Cell Mol Gastroenterol Hepatol. 13:257. PubMed
  34. Kuhn NF, et al. 2020. Nat Commun. 4.74375. PubMed
  35. Celik H, et al. 2021. Cancer Discov. 11:3126. PubMed
  36. Sharma D, et al. 2022. Commun Biol. 5:479. PubMed
  37. Reddy A, et al. 2020. Cell. 183:62. PubMed
  38. Payne K, et al. 2016. J Leukoc Biol. 100: 625 - 635. PubMed
  39. Wagner JA, et al. 2020. Cell Rep. 31:107720. PubMed
  40. Freemerman AJ, et al. 2019. J Immunol. 202:1265. PubMed
  41. Grigoryan L, et al. 2022. NPJ Vaccines. 7:55. PubMed
  42. Pishesha N, et al. 2021. Proc Natl Acad Sci U S A. 118:. PubMed
  43. Ganguly K, et al. 2022. Front Immunol. 13:930449. PubMed
  44. Bunting MD, et al. 2022. Sci Adv. 8:eabk3327. PubMed
  45. Cortez-Toledo O, et al. 2017. PLoS One. 12(2):e0171268. PubMed
  46. Scarneo SA, et al. 2022. Sci Rep. 12:18091. PubMed
  47. Wei W, et al. 2022. mSystems. 7:e0046922. PubMed
  48. Luo J, et al. 2022. J Nanobiotechnology. 20:228. PubMed
  49. Reese B, et al. 2020. J Immunol. 205:3218. PubMed
  50. Harb H, et al. 2021. Immunity. 54(6):1186-1199.e7. PubMed
  51. Gkountela S et al. 2019. Cell. 176(1-2):98-112 . PubMed
  52. Kakizaki M, Watanabe R 2017. Neuropathology. 10.1111/neup.12386. PubMed
  53. Zeng W, et al. 2021. STAR Protocols. 2(1):100361. PubMed
  54. Chen YG, et al. 2020. Molecular Cell. 76(1):96-109. PubMed
  55. Wolf KG, et al. 2022. JVS Vasc Sci. 3:336. PubMed
  56. Anderson AE, et al. 2022. NPJ Regen Med. 7:6. PubMed
  57. Hu Y, et al. 2021. Cell Death Dis. 12:743. PubMed
  58. Li J, et al. 2022. Nat Commun. 13:4032. PubMed
  59. Alam Z, et al. 2020. Cell Rep. 107825:31. PubMed
  60. Gubin MM, et al. 2018. Cell. 175:1014. PubMed
  61. Di Mitri D, et al. 2019. Cell Rep. 28:2156. PubMed
  62. Bhattacharya A, et al. 2018. Neuropsychopharmacology. 43:2586. PubMed
  63. Mohamed E, et al. 2020. Immunity. 52(4):668-682.e7.. PubMed
  64. Dulken BW, et al. 2019. Nature. 571:205. PubMed
  65. Gyoneva S, et al. 2019. Life Sci Alliance. 2:e201900453. PubMed
  66. Zhang Y, et al. 2019. Nat Commun. 10:3667. PubMed
  67. Terp MG, et al. 2021. Mol Oncol. 15:3299. PubMed
  68. Myers DR, et al. 2020. Front Immunol. 11:576310. PubMed
  69. Ovadya Y, et al. 2018. Nat Commun. 9:5435. PubMed
  70. Kang YH, et al. 2019. Nat Commun. 10:912. PubMed
  71. McDonald LT, et al. 2017. PLoS One. 12:e0180724. PubMed
  72. Olson CA, et al. 2021. Cell Host Microbe. 29:1378. PubMed
  73. Ren J, et al. 2021. JCI Insight. 6:. PubMed
  74. Harb H, et al. 2020. Nat Immunol. 1359:21. PubMed
  75. Berrien–Elliott MM, et al. 2020. Immunity. 51(3):479-490. PubMed
  76. Chen M, et al. 2019. Cell Stem Cell. 25:501. PubMed
  77. Clemente–Casares X, et al. 2017. Immunity. 47:974. PubMed
  78. Bennion BG, et al. 2019. J Virol. 93. PubMed
  79. Zhu YP et al. 2018. Cell reports. 24(9):2329-2341 . PubMed
  80. Pavelko K, et al. 2017. Front Immunol. . 10.3389/fimmu.2017.01532. PubMed
  81. Molgora M, et al. 2020. Cell. 182:886. PubMed
  82. Fedele C, et al. 2021. J Exp Med. 218: . PubMed
  83. Yin X, et al. 2020. Cell Rep. 33:108278. PubMed
  84. Becattini S, et al. 2021. Cell Host Microbe. 29(3):378-393.e5. PubMed
  85. Dai X, et al. 2021. Molecular Cell. 81(11):2317-2331.e6. PubMed
  86. Hong JP, et al. 2020. Cell Reports Medicine. 1(3):100035. PubMed
  87. Teng F, et al. 2021. Cell Rep. 37:110051. PubMed
  88. Li H, et al. 2022. iScience. 25:104481. PubMed
  89. Dubrot J, et al. 2021. Immunity. 54(3):571-585.e6. PubMed
  90. Castillo–Azofeifa D, et al. 2019. EMBO J. 38:e99984. PubMed
  91. Lee A, et al. 2022. Nat Commun. 13:549. PubMed
  92. Perez-Cruz M, et al. 2021. PLoS One. e0236216:16. PubMed
  93. Li Z et al. 2018. Immunity. 49(4):640-653 . PubMed
  94. Lloyd AF, et al. 2019. Nat Neurosci. 1.643055556. PubMed
  95. Shao Y, et al. 2019. Stem Cells. 37:1331. PubMed
  96. Avgustinova A, et al. 2021. Cell Stem Cell. . PubMed
  97. Kuhn NF et al. 2019. Cancer cell. 35(3):473-488 . PubMed
RRID
AB_2562341 (BioLegend Cat. No. 103139) AB_2650656 (BioLegend Cat. No. 103155) AB_2562342 (BioLegend Cat. No. 103140)

Antigen Details

Structure
Protein tyrosine phosphatase (PTP) family, 180-240 kD
Distribution

All hematopoietic cells except mature erythrocytes and platelets

Function
Phosphatase, T and B cell activation
Ligand/Receptor
Galectin-1, CD2, CD3, CD4, TCR, CD22, Thy-1
Cell Type
B cells, Dendritic cells, Mesenchymal Stem Cells, Tregs
Biology Area
Cell Biology, Immunology, Inhibitory Molecules, Innate Immunity, Neuroscience, Neuroscience Cell Markers, Stem Cells
Molecular Family
CD Molecules
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Trowbridge IS, et al. 1993. Annu. Rev. Immunol. 12:85.
3. Kishihara K, et al. 1993. Cell 74:143.
4. Pulido R, et al. 1988. J. Immunol. 140:3851.

Gene ID
19264 View all products for this Gene ID
UniProt
View information about CD45 on UniProt.org

联系我们

TEL：021-34661275 　点击拨打热线