Ultra-LEAF™ Purified anti-mouse CD80 Antibod-上海雅吉生物科技有限公司

规格	价格	库存
100mg	￥ 47362	1

Product Details

Verified Reactivity

Mouse

Reported Reactivity

Dog

Antibody Type

Monoclonal

Host Species

Armenian Hamster

Immunogen

CHO cell line transfected with mouse B7 (CD80)

Formulation

0.2 ?m filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is <0.01 EU/?g of the protein (<0.001 ng/?g of the protein) as determined by the LAL test.

Preparation

The Ultra-LEAF? (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.

Concentration

The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF? solution contains no preservative; handle under aseptic conditions.

Application

FC - Quality tested
Block, IHC-F, IP - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation², in vitro and in vivo blocking of CTLA-4 Ig to CD80 by blocking costimulation of T cells by activated B cells^2-4, and immunohistochemical staining of acetone-fixed frozen sections^1,4. The Ultra-LEAF? purified antibody (Endotoxin < 0.01 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. Nos. 104747-104752).

Application References (PubMed link indicates BioLegend citation)

Harlan DM, et al. 1994. P. Natl. Acad. Sci. USA 91:3137. (IHC)
Razi-Wolf Z, et al. 1992. P. Natl. Acad. Sci. USA 89:4210. (Block, IP)
Hathcock KS, et al. 1994. J. Exp. Med. 180:631. (Block)
Herold KC, et al. 1997. J. Immunol. 158:984. (Block, IHC)
Ma XT, et al. 2006. Cancer Res. 66:1169.
Andoniou CE, et al. 2005. Nature Immunology 6:1011. (FC)
Lawson BR, et al. 2007. J. Immunol. 178:5366.
Turnquist HR, et al. 2007. J. Immunol. 178:7018.
Misra RS, et al. 2010. J. Exp Med. 207:1775. PubMed
del Rio ML, et al. 2011. Transpl. Int. 24:501. (FC) PubMed
Philipsen L, et al. 2013. Mol Cell Proteomics. 12:2551. PubMed

RRID

AB_2819801 (BioLegend Cat. No. 104747) AB_2819802 (BioLegend Cat. No. 104748) AB_2819803 (BioLegend Cat. No. 104749) AB_2819804 (BioLegend Cat. No. 104750) AB_2819805 (BioLegend Cat. No. 104751) AB_2819806 (BioLegend Cat. No. 104752)

Antigen Details

Structure: Ig superfamily, 60 kD
Distribution: Macrophages, activated B cells and T cells, dendritic cells
Function: T cell costimulation
Ligand/Receptor: CD28 (stimulatory), CD152(CTLA4) (inhibitory)
Cell Type: B cells, Dendritic cells, Macrophages, T cells, Tregs
Biology Area: Cell Biology, Costimulatory Molecules, Immunology, Neuroscience, Neuroscience Cell Markers
Molecular Family: CD Molecules, Immune Checkpoint Receptors
Antigen References: 1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Linsley PS, et al. 1991. J. Exp. Med. 174:561.
3. Salomon B, et al. 2001. Annu. Rev. Immunol. 19:225.
Gene ID: 12519 View all products for this Gene ID
UniProt: View information about CD80 on UniProt.org

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