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首页 > 抗体蛋白 > 抗体
Purified anti-mouse CD80 Antibody
产品名称：
Purified anti-mouse CD80 Antibody
产品类别：
抗体
产品编号：
104702
产品应用：
104702
[价格]
规格 价格 库存
500µg ￥ 1932 1

产品详情

Product Details

Verified Reactivity
Mouse
Reported Reactivity
Dog
Antibody Type
Monoclonal
Host Species
Armenian Hamster
Immunogen
CHO cell line transfected with mouse B7 (CD80)
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
Block, IHC-F, IP - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation2, in vitro and in vivo blocking of CTLA-4 Ig to CD80 by blocking costimulation of T cells by activated B cells2-4, and immunohistochemical staining of acetone-fixed frozen sections1,4. The Ultra-LEAF? purified antibody (Endotoxin < 0.01 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. Nos. 104747-104752).

Application References

(PubMed link indicates BioLegend citation)
  1. Harlan DM, et al. 1994. P. Natl. Acad. Sci. USA 91:3137. (IHC)
  2. Razi-Wolf Z, et al. 1992. P. Natl. Acad. Sci. USA 89:4210. (Block, IP)
  3. Hathcock KS, et al. 1994. J. Exp. Med. 180:631. (Block)
  4. Herold KC, et al. 1997. J. Immunol. 158:984. (Block, IHC)
  5. Ma XT, et al. 2006. Cancer Res. 66:1169.
  6. Andoniou CE, et al. 2005. Nature Immunology 6:1011. (FC)
  7. Lawson BR, et al. 2007. J. Immunol. 178:5366.
  8. Turnquist HR, et al. 2007. J. Immunol. 178:7018.
  9. Misra RS, et al. 2010. J. Exp Med. 207:1775. PubMed
  10. del Rio ML, et al. 2011. Transpl. Int. 24:501. (FC) PubMed
  11. Philipsen L, et al. 2013. Mol Cell Proteomics. 12:2551. PubMed
Product Citations
  1. Jayachandran R, et al. 2019. Immunity. 50:152. PubMed
  2. Hutton C, et al. 2021. Cancer Cell. 39:1227. PubMed
  3. Paterson MJ, et al. 2020. PLoS Pathog. 16:e1008733. PubMed
  4. Wan Mohd Zawawi WFA, et al. 2021. Sci Rep. 11:10278. PubMed
  5. Khan KA, et al. 2020. NPJ Breast Cancer. 6:29. PubMed
  6. Kouwenberg M, et al. 2020. PLoS One. 15:e0230835. PubMed
  7. Niss Arfelt K, et al. 2017. Blood. 129:866. PubMed
  8. Khan KA, et al. 2020. NPJ Breast Cancer. 6:29. PubMed
  9. Chen L, et al. 2020. Front Immunol. 11:584458. PubMed
  10. Zhao L, et al. 2018. Nat Med. 24:1536. PubMed
  11. Forsyth KS, et al. 2020. PLoS Pathog. 16:e1008685. PubMed
  12. Ruer-Laventie J, et al. 2020. Bio Protoc. e3531:10. PubMed
  13. Hu G, et al. 2021. Nat Commun. 12:773. PubMed
  14. Murakami R, et al. 2013. PLoS One. 8:73270. PubMed
  15. Chen HW, et al. 2021. Biomedicines. 9:. PubMed
  16. Wu J, et al. 2017. Am J Pathol. 10.1016/j.ajpath.2016.12.021. PubMed
  17. Hammer A, et al. 2017. Front Immunol. . 10.3389/fimmu.2017.01922. PubMed
  18. Song K, et al. 2017. Oncotarget. 8:38554. PubMed
  19. Akiel M, et al. 2017. Cancer Res. 77:4014. PubMed
  20. Roussey JA, et al. 2017. J Immunol. 199:3535. PubMed
  21. Scortegagna M, et al. 2020. Nat Commun. 11:99. PubMed
  22. Lou F, et al. 2020. Immunity. 53(1):204-216.e10. PubMed
  23. Andrews AR, et al. 2019. Cancer Cell Int. 0.955555556. PubMed
  24. Diao J, et al. 2018. J Immunol. 201:1306. PubMed
  25. Khameneh HJ, et al. 2017. J Immunol. 198:196. PubMed
  26. Klinger M, et al. 2007. Am J Physiol Regul Integr Comp Physiol. 293:R677. PubMed
  27. Prabakaran T, et al. 2021. EBioMedicine. 66:103314. PubMed
RRID
AB_313123 (BioLegend Cat. No. 104702)

Antigen Details

Structure
Ig superfamily, 60 kD
Distribution

Macrophages, activated B cells and T cells, dendritic cells

Function
T cell costimulation
Ligand/Receptor
CD28 (stimulatory), CD152(CTLA4) (inhibitory)
Cell Type
B cells, Dendritic cells, Macrophages, T cells, Tregs
Biology Area
Cell Biology, Costimulatory Molecules, Immunology, Neuroscience, Neuroscience Cell Markers
Molecular Family
CD Molecules, Immune Checkpoint Receptors
Antigen References

1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Linsley PS, et al. 1991. J. Exp. Med. 174:561.
3. Salomon B, et al. 2001. Annu. Rev. Immunol. 19:225.

Gene ID
12519 View all products for this Gene ID
UniProt
View information about CD80 on UniProt.org

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