Purified anti-mouse CD80 (Maxpar® Ready) Antib-上海雅吉生物科技有限公司

规格	价格	库存
100µg	￥ 1484	1

Product Details

Verified Reactivity

Mouse

Reported Reactivity

Dog

Antibody Type

Monoclonal

Host Species

Armenian Hamster

Immunogen

CHO cell line transfected with mouse B7 (CD80)

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA.

Preparation

The antibody was purified by affinity chromatography.

Concentration

1.0 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C.

Application

FC - Quality tested
CyTOF? - Verified

Recommended Usage

This product is suitable for use with the?Maxpar? Metal Labeling Kits.?For metal labeling using Maxpar? Ready antibodies,?proceed directly to the step to Partially Reduce the Antibody by adding 100 ?l of Maxpar? Ready antibody to 100 ?l of 4 mM TCEP-R in a 50 kDa filter and continue with the protocol. Always refer to the latest version of Maxpar? User Guide when conjugating Maxpar? Ready antibodies.

Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation², in vitro and in vivo blocking of CTLA-4 Ig to CD80 by blocking costimulation of T cells by activated B cells^2-4, and immunohistochemical staining of acetone-fixed frozen sections^1,4. The Ultra-LEAF? purified antibody (Endotoxin < 0.01 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. Nos. 104747-104752).

Additional Product Notes

Maxpar? is a registered trademark of Standard BioTools Inc.

Application References (PubMed link indicates BioLegend citation)

Harlan DM, et al. 1994. P. Natl. Acad. Sci. USA 91:3137. (IHC)
Razi-Wolf Z, et al. 1992. P. Natl. Acad. Sci. USA 89:4210. (Block, IP)
Hathcock KS, et al. 1994. J. Exp. Med. 180:631. (Block)
Herold KC, et al. 1997. J. Immunol. 158:984. (Block, IHC)
Ma XT, et al. 2006. Cancer Res. 66:1169.
Andoniou CE, et al. 2005. Nature Immunology 6:1011. (FC)
Lawson BR, et al. 2007. J. Immunol. 178:5366.
Turnquist HR, et al. 2007. J. Immunol. 178:7018.
Misra RS, et al. 2010. J. Exp Med. 207:1775. PubMed
del Rio ML, et al. 2011. Transpl. Int. 24:501. (FC) PubMed
Philipsen L, et al. 2013. Mol Cell Proteomics. 12:2551. PubMed

Product Citations

Martinez LE, et al. 2020. J Virol. 94:00:00. PubMed
Wei SC et al. 2017. Cell. 170(6):1120-1133 . PubMed

RRID

AB_2563763 (BioLegend Cat. No. 104735)

Antigen Details

Structure: Ig superfamily, 60 kD
Distribution: Macrophages, activated B cells and T cells, dendritic cells
Function: T cell costimulation
Ligand/Receptor: CD28 (stimulatory), CD152(CTLA4) (inhibitory)
Cell Type: B cells, Dendritic cells, Macrophages, T cells, Tregs
Biology Area: Cell Biology, Costimulatory Molecules, Immunology, Neuroscience, Neuroscience Cell Markers
Molecular Family: CD Molecules, Immune Checkpoint Receptors
Antigen References: 1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Linsley PS, et al. 1991. J. Exp. Med. 174:561.
3. Salomon B, et al. 2001. Annu. Rev. Immunol. 19:225.
Gene ID: 12519 View all products for this Gene ID
UniProt: View information about CD80 on UniProt.org

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