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PE/Cyanine7 anti-mouse CD80 Antibody
产品名称：
PE/Cyanine7 anti-mouse CD80 Antibody
产品类别：
抗体
产品编号：
104733
产品应用：
104733
[价格]
规格 价格 库存
25µg ￥ 1624 1

产品详情

Product Details

Verified Reactivity
Mouse
Reported Reactivity
Dog
Antibody Type
Monoclonal
Host Species
Armenian Hamster
Immunogen
CHO cell line transfected with mouse B7 (CD80)
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with PE/Cyanine7 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation2, in vitro and in vivo blocking of CTLA-4 Ig to CD80 by blocking costimulation of T cells by activated B cells2-4, and immunohistochemical staining of acetone-fixed frozen sections1,4. The Ultra-LEAF? purified antibody (Endotoxin < 0.01 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. Nos. 104747-104752).

Additional Product Notes
BioLegend is in the process of converting the name PE/Cy7 to PE/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance from our PE/Cyanine7 products. Please contact Technical Service if you have any questions.
Application References

(PubMed link indicates BioLegend citation)
  1. Harlan DM, et al. 1994. P. Natl. Acad. Sci. USA 91:3137. (IHC)
  2. Razi-Wolf Z, et al. 1992. P. Natl. Acad. Sci. USA 89:4210. (Block, IP)
  3. Hathcock KS, et al. 1994. J. Exp. Med. 180:631. (Block)
  4. Herold KC, et al. 1997. J. Immunol. 158:984. (Block, IHC)
  5. Ma XT, et al. 2006. Cancer Res. 66:1169.
  6. Andoniou CE, et al. 2005. Nature Immunology 6:1011. (FC)
  7. Lawson BR, et al. 2007. J. Immunol. 178:5366.
  8. Turnquist HR, et al. 2007. J. Immunol. 178:7018.
  9. Misra RS, et al. 2010. J. Exp Med. 207:1775. PubMed
  10. del Rio ML, et al. 2011. Transpl. Int. 24:501. (FC) PubMed
  11. Philipsen L, et al. 2013. Mol Cell Proteomics. 12:2551. PubMed
Product Citations
  1. Zenke S, et al. 2022. Nat Commun. 13:6459. PubMed
  2. McNamara HA, et al. 2020. Cell Host Microbe. 572:28. PubMed
  3. Cullis J, et al. 2017. Cancer Immunol Res. 5:182. PubMed
  4. Padonou F, et al. 2022. EMBO Rep. 23:e53576. PubMed
  5. Chen J, et al. 2022. J Nanobiotechnology. 20:283. PubMed
  6. Paraiso HC, et al. 2018. J Neuroinflammation. 0.694444444. PubMed
  7. Teng ZX, et al. 2022. J Inflamm Res. 15:3187. PubMed
  8. Wang S, et al. 2018. Biomaterials. 187:28:00. PubMed
  9. Cheng K, et al. 2021. Nat Commun. 12:2041. PubMed
  10. Utz B, et al. 2020. Sci Rep. 10:18733. PubMed
  11. Akazawa S, et al. 2021. Diabetologia. 64:878. PubMed
  12. Haikala HM, et al. 2019. Nat Commun. 10:620. PubMed
  13. Zhao X, et al. 2022. Nat Protoc. 17:2240. PubMed
  14. Deerhake ME, et al. 2021. Immunity. 54(3):484-498.e8. PubMed
  15. Germundson DL, et al. 2022. Front Allergy. 3:870513. PubMed
  16. Ding X, et al. 2019. J Am Heart Assoc. 8:e011211. PubMed
  17. Ishikura N, et al. 2022. Oncol Rep. 47:. PubMed
  18. Wang X, et al. 2020. Nat Commun. 4.505555556. PubMed
  19. Guo S, et al. 2021. Sci Rep. 11:23745. PubMed
  20. Kiyohara H, et al. 2018. Cell Mol Gastroenterol Hepatol. 7:135. PubMed
  21. Zhao Y, et al. 2020. Immunity. 51(6):1059-1073.e9.. PubMed
  22. Zhang W, et al. 2021. Front Immunol. 12:768435. PubMed
  23. Wang R, et al. 2022. J Immunother Cancer. 10:. PubMed
  24. Siolas D, et al. 2021. Cell Reports. 36(8):109578. PubMed
  25. Li H, et al. 2022. iScience. 25:104481. PubMed
  26. Guo Y, et al. 2021. Nat Immunol. 22:746. PubMed
RRID
AB_2563112 (BioLegend Cat. No. 104733) AB_2563113 (BioLegend Cat. No. 104734)

Antigen Details

Structure
Ig superfamily, 60 kD
Distribution

Macrophages, activated B cells and T cells, dendritic cells

Function
T cell costimulation
Ligand/Receptor
CD28 (stimulatory), CD152(CTLA4) (inhibitory)
Cell Type
B cells, Dendritic cells, Macrophages, T cells, Tregs
Biology Area
Cell Biology, Costimulatory Molecules, Immunology, Neuroscience, Neuroscience Cell Markers
Molecular Family
CD Molecules, Immune Checkpoint Receptors
Antigen References

1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Linsley PS, et al. 1991. J. Exp. Med. 174:561.
3. Salomon B, et al. 2001. Annu. Rev. Immunol. 19:225.

Gene ID
12519 View all products for this Gene ID
UniProt
View information about CD80 on UniProt.org

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