Brilliant Violet 711™ anti-mouse CD80 Antibo-上海雅吉生物科技有限公司

规格	价格	库存
50µg	￥ 4830	1

Product Details

Verified Reactivity

Mouse

Reported Reactivity

Dog

Antibody Type

Monoclonal

Host Species

Armenian Hamster

Immunogen

CHO cell line transfected with mouse B7 (CD80)

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).

Preparation

The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 711? under optimal conditions.

Concentration

0.2 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 711? excites at 405 nm and emits at 711 nm. The bandpass filter 710/50 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 711? is a trademark of Sirigen Group Ltd.

Learn more about Brilliant Violet?.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.

Excitation Laser

Violet Laser (405 nm)

Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation², in vitro and in vivo blocking of CTLA-4 Ig to CD80 by blocking costimulation of T cells by activated B cells^2-4, and immunohistochemical staining of acetone-fixed frozen sections^1,4. The Ultra-LEAF? purified antibody (Endotoxin < 0.01 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. Nos. 104747-104752).

Application References (PubMed link indicates BioLegend citation)

Harlan DM, et al. 1994. P. Natl. Acad. Sci. USA 91:3137. (IHC)
Razi-Wolf Z, et al. 1992. P. Natl. Acad. Sci. USA 89:4210. (Block, IP)
Hathcock KS, et al. 1994. J. Exp. Med. 180:631. (Block)
Herold KC, et al. 1997. J. Immunol. 158:984. (Block, IHC)
Ma XT, et al. 2006. Cancer Res. 66:1169.
Andoniou CE, et al. 2005. Nature Immunology 6:1011. (FC)
Lawson BR, et al. 2007. J. Immunol. 178:5366.
Turnquist HR, et al. 2007. J. Immunol. 178:7018.
Misra RS, et al. 2010. J. Exp Med. 207:1775. PubMed
del Rio ML, et al. 2011. Transpl. Int. 24:501. (FC) PubMed
Philipsen L, et al. 2013. Mol Cell Proteomics. 12:2551. PubMed

RRID

AB_2810338 (BioLegend Cat. No. 104743)

Antigen Details

Structure: Ig superfamily, 60 kD
Distribution: Macrophages, activated B cells and T cells, dendritic cells
Function: T cell costimulation
Ligand/Receptor: CD28 (stimulatory), CD152(CTLA4) (inhibitory)
Cell Type: B cells, Dendritic cells, Macrophages, T cells, Tregs
Biology Area: Cell Biology, Costimulatory Molecules, Immunology, Neuroscience, Neuroscience Cell Markers
Molecular Family: CD Molecules, Immune Checkpoint Receptors
Antigen References: 1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Linsley PS, et al. 1991. J. Exp. Med. 174:561.
3. Salomon B, et al. 2001. Annu. Rev. Immunol. 19:225.
Gene ID: 12519 View all products for this Gene ID
UniProt: View information about CD80 on UniProt.org

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