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Brilliant Violet 421™ anti-mouse CD80 Antibo
产品名称：
Brilliant Violet 421™ anti-mouse CD80 Antibo
产品类别：
抗体
产品编号：
104725
产品应用：
104725
[价格]
规格 价格 库存
125µL ￥ 2856 1

产品详情

Product Details

Verified Reactivity
Mouse
Reported Reactivity
Dog
Antibody Type
Monoclonal
Host Species
Armenian Hamster
Immunogen
CHO cell line transfected with mouse B7 (CD80)
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421? under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
IHC-F - Verified
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood. For immunohistochemical staining on frozen tissue sections, the suggested use is 5-10 ?g/mL. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 421? excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421? is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet?.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation2, in vitro and in vivo blocking of CTLA-4 Ig to CD80 by blocking costimulation of T cells by activated B cells2-4, and immunohistochemical staining of acetone-fixed frozen sections1,4. The Ultra-LEAF? purified antibody (Endotoxin < 0.01 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. Nos. 104747-104752).

Application References

(PubMed link indicates BioLegend citation)
  1. Harlan DM, et al. 1994. P. Natl. Acad. Sci. USA 91:3137. (IHC)
  2. Razi-Wolf Z, et al. 1992. P. Natl. Acad. Sci. USA 89:4210. (Block, IP)
  3. Hathcock KS, et al. 1994. J. Exp. Med. 180:631. (Block)
  4. Herold KC, et al. 1997. J. Immunol. 158:984. (Block, IHC)
  5. Ma XT, et al. 2006. Cancer Res. 66:1169.
  6. Andoniou CE, et al. 2005. Nature Immunology 6:1011. (FC)
  7. Lawson BR, et al. 2007. J. Immunol. 178:5366.
  8. Turnquist HR, et al. 2007. J. Immunol. 178:7018.
  9. Misra RS, et al. 2010. J. Exp Med. 207:1775. PubMed
  10. del Rio ML, et al. 2011. Transpl. Int. 24:501. (FC) PubMed
  11. Philipsen L, et al. 2013. Mol Cell Proteomics. 12:2551. PubMed
Product Citations
  1. Zenke S, et al. 2022. Nat Commun. 13:6459. PubMed
  2. McNamara HA, et al. 2020. Cell Host Microbe. 572:28. PubMed
  3. Chakrabarti J, et al. 2018. Oncotarget. 9:37439. PubMed
  4. Go DM, et al. 2021. Cell Mol Gastroenterol Hepatol. 12:715. PubMed
  5. White A, et al. 2014. J Immunol. 192:2659. PubMed
  6. Super M, et al. 2021. Nat Biomed Eng. Online ahead of print. PubMed
  7. Zenke S, et al. 2020. Immunity. 52(2):313-327. PubMed
  8. Desanti G, et al. 2012. J Immunol. 189:5519. PubMed
  9. Venturutti L, et al. 2020. Cell. 182(2):297-316.e27. PubMed
  10. De Santi C, et al. 2022. Mol Ther Nucleic Acids. 29:643. PubMed
  11. Cowan J, et al. 2014. J Immunol. 193:1204. PubMed
  12. Abbott RK, et al. 2018. Immunity. 48:133. PubMed
  13. Ganguly S, et al. 2021. Cell Mol Gastroenterol Hepatol. 12:891. PubMed
  14. Hasegawa T, et al. 2020. Sci Rep. 10:13480. PubMed
  15. Kumagai S, et al. 2020. Immunity. 53(1):187-203.e8. PubMed
  16. Chen Y, et al. 2022. Nat Commun. 13:4468. PubMed
  17. Zhang L, et al. 2020. Cell. 442:181. PubMed
  18. Aglas L, et al. 2018. J Allergy Clin Immunol. 142:984. PubMed
RRID
AB_10900989 (BioLegend Cat. No. 104725) AB_2561445 (BioLegend Cat. No. 104726)

Antigen Details

Structure
Ig superfamily, 60 kD
Distribution

Macrophages, activated B cells and T cells, dendritic cells

Function
T cell costimulation
Ligand/Receptor
CD28 (stimulatory), CD152(CTLA4) (inhibitory)
Cell Type
B cells, Dendritic cells, Macrophages, T cells, Tregs
Biology Area
Cell Biology, Costimulatory Molecules, Immunology, Neuroscience, Neuroscience Cell Markers
Molecular Family
CD Molecules, Immune Checkpoint Receptors
Antigen References

1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Linsley PS, et al. 1991. J. Exp. Med. 174:561.
3. Salomon B, et al. 2001. Annu. Rev. Immunol. 19:225.

Gene ID
12519 View all products for this Gene ID
UniProt
View information about CD80 on UniProt.org

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