Alexa Fluor® 647 anti-mouse CD80 Antibody -上海雅吉生物科技有限公司

规格	价格	库存
25µg	￥ 1414	1

Product Details

Verified Reactivity

Mouse

Reported Reactivity

Dog

Antibody Type

Monoclonal

Host Species

Armenian Hamster

Immunogen

CHO cell line transfected with mouse B7 (CD80)

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 647 under optimal conditions.

Concentration

0.5 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested
IHC-F - Verified

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 ?g per million cells in 100 ?l volume.?For immunohistochemistry on frozen tissue sections, a concentration range of 2.5 - 5.0 ?g/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor? 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm.

Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.

View full statement regarding label licenses

Excitation Laser

Red Laser (633 nm)

Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation², in vitro and in vivo blocking of CTLA-4 Ig to CD80 by blocking costimulation of T cells by activated B cells^2-4, and immunohistochemical staining of acetone-fixed frozen sections^1,4. The Ultra-LEAF? purified antibody (Endotoxin < 0.01 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. Nos. 104747-104752).

Application References (PubMed link indicates BioLegend citation)

Harlan DM, et al. 1994. P. Natl. Acad. Sci. USA 91:3137. (IHC)
Razi-Wolf Z, et al. 1992. P. Natl. Acad. Sci. USA 89:4210. (Block, IP)
Hathcock KS, et al. 1994. J. Exp. Med. 180:631. (Block)
Herold KC, et al. 1997. J. Immunol. 158:984. (Block, IHC)
Ma XT, et al. 2006. Cancer Res. 66:1169.
Andoniou CE, et al. 2005. Nature Immunology 6:1011. (FC)
Lawson BR, et al. 2007. J. Immunol. 178:5366.
Turnquist HR, et al. 2007. J. Immunol. 178:7018.
Misra RS, et al. 2010. J. Exp Med. 207:1775. PubMed
del Rio ML, et al. 2011. Transpl. Int. 24:501. (FC) PubMed
Philipsen L, et al. 2013. Mol Cell Proteomics. 12:2551. PubMed

Product Citations

Mathur R, et al. 2019. Mucosal Immunol. 12:612. PubMed
Lauterbach MA, et al. 2020. Immunity. 51(6):997-1011.e7.. PubMed
Hu HJ, et al. 2020. Cell Death Dis. 1.168055556. PubMed
Boccasavia VL, et al. 2021. Cell Reports. 34(11):108861. PubMed
Shan M et al. 2018. Immunity. 49(4):709-724 . PubMed
Jiang Y, et al. 2020. Adv Mater. 32:e2001808. PubMed
Misra R, et al. 2010. J Exp Med. 207:1775. PubMed

RRID

AB_492825 (BioLegend Cat. No. 104717) AB_492824 (BioLegend Cat. No. 104718)

Antigen Details

Structure: Ig superfamily, 60 kD
Distribution: Macrophages, activated B cells and T cells, dendritic cells
Function: T cell costimulation
Ligand/Receptor: CD28 (stimulatory), CD152(CTLA4) (inhibitory)
Cell Type: B cells, Dendritic cells, Macrophages, T cells, Tregs
Biology Area: Cell Biology, Costimulatory Molecules, Immunology, Neuroscience, Neuroscience Cell Markers
Molecular Family: CD Molecules, Immune Checkpoint Receptors
Antigen References: 1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Linsley PS, et al. 1991. J. Exp. Med. 174:561.
3. Salomon B, et al. 2001. Annu. Rev. Immunol. 19:225.
Gene ID: 12519 View all products for this Gene ID
UniProt: View information about CD80 on UniProt.org

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