Purified anti-γ-Catenin Antibody
- 产品名称：
- Purified anti-γ-Catenin Antibody
- 产品类别：
- 抗体
- 产品编号：
- 946401
- 产品应用：
- 946401
- Verified Reactivity
- Human, Mouse
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Full-length recombinant chicken γ-catenin
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
IHC-P, ICC, ICFC - Verified
- Recommended Usage
Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.1 - 1.0 ?g/mL. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 5.0 - 25.0??g/mL is suggested. For immunocytochemistry, a concentration range of 5.0?μg/mL is recommended. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25??g per million cells in 100 ?L volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
This clone was tested for ICC on MCF7 (positive control) and MOLT-4 (negative control) cells using multiple fixation and permeabilization conditions. A brief summary of these results is listed below:
- methanol only: plasma membrane associated staining in MCF7 cells, no staining in MOLT-4 cells. Moderate nuclear background staining observed in MCF7 cells.
? - 4% PFA plus Triton X-100: plasma membrane associated staining in MCF7 cells, no staining in MOLT-4 cells. Low nuclear background staining observed in MCF7 cells.
? - 4% PFA plus methanol: 4% PFA plus Triton X-100: plasma membrane associated staining in MCF7 cells, no staining in MOLT-4 cells. Low nuclear background staining observed in MCF7 cells.
- RRID
- AB_2894532 (BioLegend Cat. No. 946401) AB_2894532 (BioLegend Cat. No. 946402)
- Structure
- γ-catenin is a 745 amino acid protein with a predicted molecular weight of 82 kD.
- Distribution
-
Ubiquitously expressed/Cytoskeleton and plasma membrane associated
- Function
- Cell to cell adhesion, cell signaling
- Biology Area
- Cell Adhesion, Cell Biology, Cell Motility/Cytoskeleton/Structure, Signal Transduction
- Antigen References
-
- Zhurinksy J, et al. 2000. J?Cell?Sci. 18:3127.
- Mahendram S,?et al. 2013.?PLoS ONE.?8:e65320.
- Aktary Z, et al. 2017. Oncotarget. 8:32270.
- Gene ID
- 3728 View all products for this Gene ID
- UniProt
- View information about gamma-Catenin on UniProt.org
