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Purified anti-APEX1 Antibody
产品名称:
Purified anti-APEX1 Antibody
产品类别:
抗体
产品编号:
605051
产品应用:
605051
[价格]
规格 价格 库存
25µg ¥ 1652 1

产品详情

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Full-length recombinant human APEX1 protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
IHC-P, ICC - Verified

Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.25 - 1.0 ?g/mL. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration of 5.0 ?g/mL is suggested. For immunocytochemistry, a concentration range of 5.0 - 10.0 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

When using this clone for ICC, we recommend using PFA + methanol or methanol only fix-perm conditions. We do not recommend using PFA + Triton X-100 due to non-specific staining.

When testing this clone for ICC, this antibody produced filamentous, extranuclear staining in some cells. This pattern was also observed with a control antibody generated using a different APEX1 immunogen, consistent with this staining being APEX1 dependent.

RRID
AB_2910484 (BioLegend Cat. No. 605051) AB_2910484 (BioLegend Cat. No. 605052)

Antigen Details

Structure
APEX1 is a 318 amino acid protein with a predicted molecular weight of ~36 kD.
Distribution

Nucleus/Ubiquitously expressed

Function
DNA repair enzyme
Biology Area
Cell Biology, DNA Repair/Replication
Molecular Family
Enzymes and Regulators
Antigen References
  1. Fishel ML, et al. 2008. DNA Repair. 7:177-86.
  2. Kim MH, et al. 2013. J?Clin?Invest. 123:3211-3230.
  3. Thakur, et al. 2014. Exp?Mol?Med.?46:e106.

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Gene ID
328 View all products for this Gene ID
UniProt
View information about APEX1 on UniProt.org

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