Purified anti-APEX1 Antibody
- 产品名称:
- Purified anti-APEX1 Antibody
- 产品类别:
- 抗体
- 产品编号:
- 605051
- 产品应用:
- 605051
- Verified Reactivity
- Human, Mouse
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Full-length recombinant human APEX1 protein
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
IHC-P, ICC - Verified
- Recommended Usage
Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.25 - 1.0 ?g/mL. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration of 5.0 ?g/mL is suggested. For immunocytochemistry, a concentration range of 5.0 - 10.0 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
When using this clone for ICC, we recommend using PFA + methanol or methanol only fix-perm conditions. We do not recommend using PFA + Triton X-100 due to non-specific staining.
When testing this clone for ICC, this antibody produced filamentous, extranuclear staining in some cells. This pattern was also observed with a control antibody generated using a different APEX1 immunogen, consistent with this staining being APEX1 dependent.
- RRID
- AB_2910484 (BioLegend Cat. No. 605051) AB_2910484 (BioLegend Cat. No. 605052)
- Structure
- APEX1 is a 318 amino acid protein with a predicted molecular weight of ~36 kD.
- Distribution
-
Nucleus/Ubiquitously expressed
- Function
- DNA repair enzyme
- Biology Area
- Cell Biology, DNA Repair/Replication
- Molecular Family
- Enzymes and Regulators
- Antigen References
-
- Fishel ML, et al. 2008. DNA Repair. 7:177-86.
- Kim MH, et al. 2013. J?Clin?Invest. 123:3211-3230.
- Thakur, et al. 2014. Exp?Mol?Med.?46:e106.
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- Gene ID
- 328 View all products for this Gene ID
- UniProt
- View information about APEX1 on UniProt.org