Purified anti-mouse/rat/human FOXP3 Antibody
- 产品名称：
- Purified anti-mouse/rat/human FOXP3 Antibody
- 产品类别：
- 抗体
- 产品编号：
- 320001
- 产品应用：
- 320001
- Verified Reactivity
- Human, Mouse, Rat
- Reported Reactivity
- Cynomolgus, Rhesus, Baboon
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Full-length FOXP3 protein
- Formulation
- This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- Upon receipt, store undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
ICFC - Reported in the literature, not verified in house
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent intracellular staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 ?g per 106 cells in 100 ?l volume. For Western blotting, the suggested working dilution(s) is ≤ 5.0 ?g/ml in antibody dilution buffer. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
NOTE: For flow cytometric staining with this clone, True-Nuclear? Transcription Factor Buffer Set (Cat. No. 424401) offers improved staining and is highly recommended.
- Application References
(PubMed link indicates BioLegend citation) -
- Roncador G, et al. 2005 Eur. J. Immunol. 35:1681.
- Mayack. S,et al. 2006. J. Immunol.176:2059. J. Immunol
- Yang ZZ, et al. 2006. Blood 107:3639.
- Gavin MA, et al. 2006. P. Natl. Acad. Sci. USA 103:6659.
- Groh V, et al. 2006. Nature Immunology 7:755.
- Lewkowicz P, et al. 2006 J. Immunol. 177:7155.
- Luke PPW, et al. 2006. Amer. J. Transplant. 6(9):2023.
- Bamias G, et al. 2007. J. Immunol. 178:1809.
- Valencia X, et al. 2007. J. Immunol. 178:2579.PubMed
- Davidson TS, et al. 2007. J. Immunol. 178:4022.
- MacDonald K PA, et al. 2007. Blood doi:10.1182/blood-2007-01-067249.
- Jaffar Z, et al. 2007. J. Immunol. 179:6193.
- Müller M, et al. 2007. J. Immunol. 179:2774.
- Jordan JM,et al. 2008.Infect Human. 76:3717. PubMed
- Golovina TN,et al. 2008. J. Immunol. 181:2855. PubMed
- Fallarino F, et al. 2009. J. Exp Med. 206:2511. PubMed
- Banham Alison, et al. 2009. Vet Immunol and Immunop 127.3-4:376-381
- Klunker S, et al. 2009. J. Exp Med. PubMed
- Haque A, et al. 2010. J. Immunol. 184:2583. PubMed
- Liu Y, et al. 2012. Food Chem Toxicol. 50:1920. PubMed
- Product Citations
-
- Berg S 2006. J Immunol. 176:2059. PubMed
- Overacre-Delgoffe AE, et al. 2017. Cell. 169:1130. PubMed
- Tan Z, et al. 2020. Mol Ther Oncolytics. 16:302. PubMed
- Zareie P, et al. 2017. J Neuroinflammation. 0.630555556. PubMed
- Wang Y, et al. 2020. Ann Transl Med. 0.935416667. PubMed
- Liu Y, et al. 2012. Food Chem Toxicol. 50:1920. PubMed
- Sattler A, et al. 2021. Am J Transplant. 21:87. PubMed
- Miyabe C, et al. 2017. Ann Rheum Dis. 76:898. PubMed
- Abdelfattah N, et al. 2022. Nat Commun. 13:767. PubMed
- Yang J, et al. 2019. Front Immunol. 2.492361111. PubMed
- Jordan J, et al. 2008. Infect Immun. 76:3717. PubMed
- RRID
- AB_439745 (BioLegend Cat. No. 320001) AB_439746 (BioLegend Cat. No. 320002)
- Structure
- Forkhead/winged-helix transcription factor family, approximately 50 kD, contains zinc finger and forkhead domains
- Distribution
-
Nuclear; expressed in T regulatory cells
- Function
- Transcription factor proposed to be a master regulatory gene in T regulatory cell development and a critical factor for immune homeostasis
- Interaction
- Interacts with DNA
- Cell Type
- Tregs
- Biology Area
- Cell Biology, Immunology, Transcription Factors
- Molecular Family
- Nuclear Markers
- Antigen References
-
1. Hori S, et al. 2003. Science 299:1057.
- Regulation
- FOXP3 is present at high levels in T regulatory cell can also be induced by T cell activation
- Gene ID
- 20371 View all products for this Gene ID 317382 View all products for this Gene ID 50943 View all products for this Gene ID
- UniProt
- View information about FOXP3 on UniProt.org
