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Purified anti-mouse I-A
产品名称:
Purified anti-mouse I-A
产品编号:
110002
产品类别:
牛ELISA试剂盒
检测样本:
110002
[价格]
规格 价格 库存
500µg ¥ 253 1

产品详情

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
CKB mouse splenocytes
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
IHC-F, IP - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation1, blocking of MHC class II - restricted CD4+ T cell responses2,3, induction of B cell differentiation4, and immunohistochemical staining5 of acetone-fixed frozen sections.

This clone does not cross react with other haplotypes (e.g., b, d, f, p, q, s).

Application References

(PubMed link indicates BioLegend citation)
  1. Oi VT, et al. 1978. Curr. Top. Microbiol. Immunol. 81:115. (IP)
  2. Formby B, et al. 1990. P. Natl. Acad. Sci. USA 87:2438.
  3. Kappler JW, et al. 1981. J. Exp. Med. 153:1198.
  4. Bishop GA, et al. 1989. P. Natl. Acad. Sci. USA 86:5933.
  5. Ardehali A, et al. 1995. Circulation 92:450. (IHC)
  6. Turnquist HR, et al. 2007. J. Immunol. 178:7018.
  7. Zhang L, et al. 2011. Lupus. 20:667. PubMed.
Product Citations
  1. Zhang L, et al. 2011. Lupus. 1.296527778. PubMed
  2. Hausmann A, et al. 2021. J Exp Med. 218:. PubMed
  3. Barroso M, et al. 2015. J Biol Chem. 290: 27101 - 27112. PubMed
RRID
AB_313459 (BioLegend Cat. No. 110002)

Antigen Details

Structure
α chain of MHC class II
Distribution

B cell and activated T cells, APCs of H-2b mice

Function
Antigen presentation
Ligand/Receptor
CD3/TCR, CD4
Cell Type
Antigen-presenting cells, B cells, T cells, Tregs
Biology Area
Immunology, Innate Immunity
Molecular Family
MHC Antigens
Antigen References

1. Watts C. 1997. Ann. Rev. Immunol. 15:821.
2. Pamer E, et al. 1998. Ann. Rev. Immunol. 16:323.

Gene ID
14960 View all products for this Gene ID
UniProt
View information about I-Ak on UniProt.org

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