Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.

Additional reported applications (for the relevant formats) include: immunofluorescence⁴, blocking^6,7,8,9, and immunohistochemistry of acetone-fixed frozen sections^{4, 11}. The LEAF? purified antibody (Endotoxin <0.1 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. No. 124303). For highly sensitive assays, we recommend Ultra-LEAF? purified antibody (Cat. No. 124318) with a lower endotoxin limit than standard LEAF? purified antibodies (Endotoxin <0.01 EU/?g).

1. Maier H, et al. 2007. J. Immunol. 178:2714.
2. Meng Q, et al. 2006. Invest. Ophthalmol. Vis. Sci. 47:4444. PubMed
3. Scarlett UK, et al. 2012. J Exp Med. 209:495. PubMed
4. Grabie N, et al. 2007. Circulation 116:2062. (IF, IHC)
5. Paterson AM, et al. 2011. J. Immunol. 187:1097.
6. Channappanavar R, et al. 2012. PLoS One 7:e39757. (Block)
7. Schreiber HA, et al. 2010. PLoS One 5:e11453. (Block) PubMed
8. Muthumani K, et al. 2011. J. Immunol. 187:2932. (Block) PubMed
9. Cripps JG, et al. 2010. Hepatology 52:1350. (Block) PubMed
10. Murakami R, et al. 2013. PLoS One. 8:73270. PubMed
11. Riella LV, et al. 2011. Am. J. Transplant 11:832-40. (IHC)
12. Lei GS, et al. 2015. Infect Immun. 83:572. PubMed

1. Sandu I, et al. 2020. Nat Commun. 11:4454. PubMed
2. Xiong W, et al. 2022. Nat Commun. 13:1700. PubMed
3. Yoon HJ, et al. 2022. Exp Mol Med. Online ahead of print. PubMed
4. Khan KA, et al. 2020. NPJ Breast Cancer. 6:29. PubMed
5. Mulder R, et al. 2017. Front Immunol. 1.464583333. PubMed
6. Fitzgerald B, et al. 2021. Cell Rep Methods. 1:. PubMed
7. Iwasaki Y, et al. 2016. PLoS One. 11: 0158282. PubMed
8. Zhang Y, et al. 2020. Oncol Lett. 1.053472222. PubMed
9. Shi J, et al. 2018. Immunity. 49:264. PubMed
10. Go DM, et al. 2021. Cell Mol Gastroenterol Hepatol. 12:715. PubMed
11. Mardomi A, et al. 2021. J Cell Physiol. 236:6328. PubMed
12. Yang M, et al. 2020. Appl Environ Microbiol. 86:00:00. PubMed
13. Lu SX, et al. 2021. Cell. . PubMed
14. Nagai Y, et al. 2019. Front Immunol. 10:174. PubMed
15. Zhang C, et al. 2021. Clin Transl Immunology. 10:e1310. PubMed
16. Li H, et al. 2021. Nat Commun. 12:2773. PubMed
17. Dichtl S, et al. 2021. Sci Adv. 7: . PubMed
18. Lv H, et al. 2020. Cell Metabolism. 33(1):110-127.e5. PubMed
19. Vackova J, et al. 2020. Int J Mol Sci. 21:00. PubMed
20. Jansen MAA, et al. 2019. Front Immunol. 1.852777778. PubMed
21. Shen Y, et al. 2022. CNS Neurosci Ther. 28:46. PubMed
22. Wang B, et al. 2022. Nat Commun. 13:3821. PubMed
23. Zhang S, et al. 2019. Mol Med Rep. 19:5377. PubMed
24. Khan KA, et al. 2020. NPJ Breast Cancer. 6:29. PubMed
25. Pauken KE, et al. 2020. Cell Reports. 31(13):107827. PubMed
26. Guo Q, et al. 2022. Nat Commun. 13:3837. PubMed
27. Wu L, et al. 2022. Theranostics. 12:842. PubMed
28. Mehta AK, et al. 2021. Nat Cancer. 2:66. PubMed
29. Zhu XG, et al. 2020. Cell Metabolism. 33(1):211-221.e6. PubMed
30. Wu Y, et al. 2021. Nat Commun. 12:2346. PubMed
31. Ramos RN, et al. 2022. Clin Transl Immunology. 11:e1392. PubMed
32. Lasso P, et al. 2020. Front Immunol. 584959:11. PubMed
33. Takano T, et al. 2020. Front Immunol. 11:1555. PubMed
34. Zuo S, et al. 2021. J Immunother Cancer. 9:. PubMed
35. Mirando AC, et al. 2020. Oncoimmunology. 9:1760685. PubMed
36. Garo LP, et al. 2019. Cell Rep. 28:3353. PubMed
37. Sheng W et al. 2018. Cell. 174(3):549-563 . PubMed
38. Habib S, et al. 2018. Infect Immun. 86:e00019. PubMed
39. Webb ER, et al. 2022. iScience. 25:104995. PubMed
40. Pathania AS, et al. 2022. Mol Ther Oncolytics. 25:308. PubMed
41. Moon S, et al. 2021. J Exp Med. 218:. PubMed
42. Shen JZ, et al. 2020. Cell. 184(2):352-369.e23. PubMed
43. van Oostrum M, et al. 2019. Nat Commun. 4.398611111. PubMed
44. Lozano-Rodríguez R, et al. 2022. Cell Rep. 38:110235. PubMed
45. Huang J, et al. 2014. J Immunol. 192:1972. PubMed
46. Lastwika K, et al. 2016. Cancer Res . 76: 227 - 238. PubMed
47. Ou YC, et al. 2020. ACS Nano. 1.035416667. PubMed
48. Campesato LF, et al. 2020. Nat Commun. 3.24375. PubMed
49. Kriegsman BA, et al. 2019. J Immunol. 203:1999. PubMed
50. Dimitrov V, et al. 2017. J Biol Chem. 292:20657. PubMed
51. Zhao Y et al. 2018. Cell reports. 24(2):379-390 . PubMed
52. Zhang X, et al. 2021. Nat Commun. 12:4536. PubMed
53. Wang J, et al. 2022. Clin Transl Med. 12:e718. PubMed
54. Apte S, et al. 2011. Int J Parasitol. 41:1285. PubMed
55. Wedekind MF, et al. 2021. iScience. 24(7):102759. PubMed
56. Zhao Y, et al. 2020. Immunity. 51(6):1059-1073.e9.. PubMed
57. Pi?ón-Zárate G, et al. 2022. Molecules. 27:. PubMed
58. Sugita S, et al. 2013. Invest Ophthalmol Vis Sci . 54:3420. PubMed
59. Liu Y, et al. 2018. Cancer Cell. 33:480. PubMed
60. Yin X, et al. 2020. Cell Rep. 33:108278. PubMed
61. Dai X, et al. 2021. Molecular Cell. 81(11):2317-2331.e6. PubMed
62. Chryplewicz A, et al. 2022. Cancer Cell. 40:1111. PubMed
63. Zhou Y, et al. 2022. Front Oncol. 12:825783. PubMed
64. Liu X, et al. 2021. Adv Sci (Weinh). 8:e2100233. PubMed
65. Nakagawa Y, et al. 2015. Immunol Lett. 167: 72-86. PubMed
66. Yamashita M, et al. 2019. Cell Stem Cell. 25:357. PubMed
67. Akhand SS, et al. 2020. Cancer Immunol Res. 8:1542. PubMed
68. Strait AA, et al. 2021. Commun Biol. 4:1005. PubMed
69. Zheng Y, et al. 2022. Transl Res. :. PubMed
70. Zhang Z, et al. 2022. J Inflamm Res. 15:5595. PubMed
71. Kim SI, et al. 2020. Molecular Cancer Therapeutics. 20(1):173-182. PubMed
72. Wang J, et al. 2020. J Hematol Oncol. 0.610416667. PubMed
73. Haile S, et al. 2011. J Immunol. 186:6822. PubMed
74. Sethuraman SN, et al. 2020. Theranostics. 10:3397. PubMed
75. Wang H, et al. 2022. J Cancer. 13:2126. PubMed
76. Lal JC, et al. 2021. Breast Cancer Res. 23:83. PubMed
77. Zuo S, et al. 2021. EBioMedicine. 64:103240. PubMed
78. Loptez-Albraitero A, et al. 2017. OncoImmunology. 10.1080/2162402X.2016.1267891. PubMed
79. Qi S, et al. 2020. Theranostics. 10:1814. PubMed

T cells, B cells, NK cells, dendritic cells, IFN-γ activated endothelial cells, and monocytes

1. Sharpe A, et al. 2007. Nat. Immunol. 8:239.
2. Dong H, et al. 1999. Nat. Med. 5:1365.
3. Freeman G, et al. 2000. J. Exp. Med. 192:1027.