Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 ?g per million cells in 100 ?L?volume or 100 ?L?of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

For in vivo and in vitro applications, we recommend to perform a pilot experiment to determine the optimal concentration to use for each particular experiment.

GoInVivo? products are guaranteed to be pathogen-free based on the IDEXX BioResearch IMPACT test via PCR. For a full listing of pathogens tested, visit the GoInVivo? Webpage.?

Additional reported applications (for the relevant formats) include: immunofluorescence⁴ and blocking^6,7,8,9, and immunohistochemistry of acetone-fixed frozen sections^{11, 12}.

View more applications data for this product in our Scientific Poster Library.

1. Maier H, et al. 2007. J. Immunol. 178:2714.
2. Meng Q, et al. 2006. Invest. Ophthalmol. Vis. Sci. 47:4444. PubMed
3. Scarlett UK, et al. 2012. J Exp Med. 209:495. PubMed
4. Grabie N, et al. 2007. Circulation 116:2062. (IF)
5. Paterson AM, et al. 2011. J. Immunol. 187:1097.
6. Channappanavar R, et al. 2012. PLoS One 7:e39757. (Block)
7. Schreiber HA, et al. 2010. PLoS One 5:e11453. (Block) PubMed
8. Muthumani K, et al. 2011. J. Immunol. 187:2932. (Block) PubMed
9. Cripps JG, et al. 2010. Hepatology 52:1350. (Block) PubMed
10. Murakami R, et al. 2013. PLoS One. 8:73270. PubMed
11. Riella LV, et al. 2011. Am. J. Transplant 11:832-40. (IHC)
12. Grabie N, et al. 2007. Circulation 116:2062-71. (IHC)
13. Lei GS, et al. 2015. Infect Immun. 83:572. PubMed

1. Bazan-Peregrino M, et al. 2021. J Immunother Cancer. 9:. PubMed
2. Chen H, et al. 2022. J Immunother Cancer. 10:. PubMed
3. Li D, et al. 2021. Mol Cancer Ther. 20:1926. PubMed
4. Li H, et al. 2021. Nat Commun. 12:2773. PubMed
5. Jaynes JM, et al. 2020. Sci Transl Med. 12:00. PubMed
6. Habib S, et al. 2018. Infect Immun. 86:e00019. PubMed
7. Janela B, et al. 2019. Immunity. 50:1069. PubMed
8. Wang C, et al. 2018. Sci Transl Med. 10. PubMed
9. Yamazaki K, et al. 2022. Sci Rep. 12:3242. PubMed

T cells, B cells, NK cells, dendritic cells, IFN-γ activated endothelial cells, and monocytes

1. Sharpe A, et al. 2007. Nat. Immunol. 8:239.
2. Dong H, et al. 1999. Nat. Med. 5:1365.
3. Freeman G, et al. 2000. J. Exp. Med. 192:1027.