产品分类/PRODUCT CLASSIFICATION
ELISA Detection - Quality tested
Each lot of this protein is quality control tested by ELISA assay. A concentration of 0.125 ?g/ml of the detection antibody was utilized to generate the example standard curve. It is recommended that each lot of reagent be titrated for optimal performance for each application.
ELISA: To measure human MIF, the purified 10C3 antibody (Cat. No. 525501) is useful as the capture antibody in a sandwich ELISA assay, when used in conjunction with the biotinylated 10C3 antibody (Cat. No. 525503) as the detection antibody.
Additional reported applications (for the relevant formats) include: functional assay1 and neutralization1.The LEAF? purified antibody (Endotoxin <0.1 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (contact our custom solutions team).
Note: For testing human MIF in serum, plasma or cell culture supernatant, BioLegend's LEGEND MAX? ELISA Kits (Cat. No. 438407 & 438408) are specially developed and recommended.
Activated T-cells, macrophages, eosinophils, epithelial cells, and endothelial cells
1. Zhou H, et al. 2009. PLoS ONE. 4:e6087.
2. Potolicchio I, et al. 2003. J. Biol. Chem. 278:30889.
3. Meyer-Siegler K & Iczkowski K. 2005. BMC Cancer. 5:73.
4. Babu SN, et al. 2012. Asian Pac. J. Cancer Prev. 13:1737.
