PE anti-Nestin Antibody -上海雅吉生物科技有限公司

Product Details

Verified Reactivity

Human

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

Fusion protein

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)

Preparation

The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.

Concentration

Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

ICFC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by intracellular flow cytometry using our True-Nuclear? Transcription Factor Staining Protocol. For flow cytometric staining, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood.

Excitation Laser

Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)

Application Notes

NOTE: For flow cytometric staining with this clone, True-Nuclear? Transcription Factor Buffer Set (Cat. No. 424401) offers improved staining and is highly recommended.

Application References (PubMed link indicates BioLegend citation)

Jozef S, et al. 2012. Biomed. Pap. Med. Fac. 156:135. (IHC)

Product Citations

Kuang YL, et al. 2019. Stem Cell Res. 37:101434. PubMed
López-Valero I, et al. 2020. Theranostics. 10:5120. PubMed
Gerace D, et al. 2021. Stem Cell Research. 53:102371. PubMed

RRID

AB_2566381 (BioLegend Cat. No. 656805) AB_2566382 (BioLegend Cat. No. 656806)

Antigen Details

Structure: 1621 amino acids with predicted molecular weight of 200-220 kD
Distribution: Cytosol
Function: Required for survival and renewal of neural progenitor cells; plays roles in angiogenesis and tumor formation
Interaction: Vimentin, alpha-internexin and FHOD3
Cell Type: Neural Stem Cells
Biology Area: Angiogenesis, Cell Biology, Cell Motility/Cytoskeleton/Structure, Immunology, Neuroscience, Neuroscience Cell Markers, Stem Cells
Molecular Family: Intermediate Filaments
Antigen References: 1. Matsuda Y, et al. 2013. World J. Gastroenterol. 19:42.
2. Matsuda Y, et al. 2012. Med. Mol. Morphol. 45:59.
3. Krupkova O Jr, et al. 2010. Neoplasma. 57:291.
4. Hombach-Klonisch S, et al. 2008. Arch. Immunol. Ther. Exp. (Warsz). 56:165.
5. Pilkington GJ, et al. 2005. Cell Prolif. 38:423.
Gene ID: 10763 View all products for this Gene ID
UniProt: View information about Nestin on UniProt.org

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