Alexa Fluor® 647 anti-Nestin Antibody -上海雅吉生物科技有限公司

Product Details

Verified Reactivity

Human

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

Fusion protein

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 647 under optimal conditions.

Concentration

0.5 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

ICC - Quality tested
IHC-P - Verified

Recommended Usage

Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a concentration range of 2.5 - 5.0 μg/ml is recommended. For immunohistochemical staining on?formalin-fixed paraffin-embedded tissue sections, a concentration range of 5.0 - 10.0 ?g/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor? 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.

Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.

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Excitation Laser

Red Laser (633 nm)

Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemistry¹.

Application References (PubMed link indicates BioLegend citation)

Jozef S, et al. 2012. Biomed. Pap. Med. Fac. 156:135. (IHC)

Product Citations

Woloshuk A, et al. 2021. Cytometry A. 99:707. PubMed

RRID

AB_2616772 (BioLegend Cat. No. 656810)

Antigen Details

Structure: 1621 amino acids with predicted molecular weight of 200-220 kD
Distribution: Cytosol
Function: Required for survival and renewal of neural progenitor cells; plays roles in angiogenesis and tumor formation
Interaction: Vimentin, alpha-internexin and FHOD3
Cell Type: Neural Stem Cells
Biology Area: Angiogenesis, Cell Biology, Cell Motility/Cytoskeleton/Structure, Immunology, Neuroscience, Neuroscience Cell Markers, Stem Cells
Molecular Family: Intermediate Filaments
Antigen References: 1. Matsuda Y, et al. 2013. World J. Gastroenterol. 19:42.
2. Matsuda Y, et al. 2012. Med. Mol. Morphol. 45:59.
3. Krupkova O Jr, et al. 2010. Neoplasma. 57:291.
4. Hombach-Klonisch S, et al. 2008. Arch. Immunol. Ther. Exp. (Warsz). 56:165.
5. Pilkington GJ, et al. 2005. Cell Prolif. 38:423.
Gene ID: 10763 View all products for this Gene ID
UniProt: View information about Nestin on UniProt.org

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